pcDNA5/FRT/TO GFP HSPA8 | Addgene 19487 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pcDNA5/FRT/TO GFP HSPA8

catalog :

19487

citations: 8

Reference
Liu F, Morderer D, Wren M, Vettleson Trutza S, Wang Y, Rabichow B, et al. Proximity proteomics of C9orf72 dipeptide repeat proteins identifies molecular chaperones as modifiers of poly-GA aggregation. Acta Neuropathol Commun. 2022;10:22 pubmed publisher
Hormazabal J, Saavedra F, Espinoza Arratia C, Martinez N, Cruces T, Alfaro I, et al. Chaperone mediated autophagy contributes to the newly synthesized histones H3 and H4 quality control. Nucleic Acids Res. 2022;50:1875-1887 pubmed publisher
Jaimon E, Tripathi A, Khurana A, Ghosh D, Sugatha J, Datta S. Binding with heat shock cognate protein HSC70 fine-tunes the Golgi association of the small GTPase ARL5B. J Biol Chem. 2021;297:101422 pubmed publisher
Chen B, Chang Y, Lin S, Yang W. Hsc70/Stub1 promotes the removal of individual oxidatively stressed peroxisomes. Nat Commun. 2020;11:5267 pubmed publisher
Liu Y, Yang E, Shi C, Mou P, Zhang B, Wu C, et al. Histone Acetyltransferase (HAT) P300/CBP Inhibitors Induce Synthetic Lethality in PTEN-Deficient Colorectal Cancer Cells through Destabilizing AKT. Int J Biol Sci. 2020;16:1774-1784 pubmed publisher
Manee M, Alharbi S, Algarni A, Alghamdi W, Altammami M, Alkhrayef M, et al. Molecular cloning, bioinformatics analysis, and expression of small heat shock protein beta-1 from Camelus dromedarius, Arabian camel. PLoS ONE. 2017;12:e0189905 pubmed publisher
Aeberhard L, Banhart S, Fischer M, Jehmlich N, Rose L, Koch S, et al. The Proteome of the Isolated Chlamydia trachomatis Containing Vacuole Reveals a Complex Trafficking Platform Enriched for Retromer Components. PLoS Pathog. 2015;11:e1004883 pubmed publisher
Hageman J, Kampinga H. Computational analysis of the human HSPH/HSPA/DNAJ family and cloning of a human HSPH/HSPA/DNAJ expression library. Cell Stress Chaperones. 2009;14:1-21 pubmed publisher

product information

Catalog Number :

19487

Product Name :

pcDNA5/FRT/TO GFP HSPA8

article :

doi	10.1007/s12192-008-0060-2
id	2277
pubmed_id	18686016

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA5/FRT/TO

backbone_mutation

backbone_origin

Invitrogen

backbone_size

5137

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Part of the HSP70 gene family Disclaimer: For cloning the various HSP genes, the Stratagene reference RNA for cDNA synthesis was used and genes were amplified from this mixture that originates from 10 different cell lines from different somatic origin and ultimately coming from 10 different individuals. This strategy was used to maximize the chance for the presence of individual HSP transcripts. Users must be aware that this mixture thus represents 20 complete haploid genomes, meaning that for each individual gene there might be polymorphisms that could potentially have functional consequences. The library was sequence verified only for the presence of the correct gene.

origin :

pi :

alt_names

HSC70, HSC71, HSP71, HSP73

cloning

clone_method	Restriction Enzyme
cloning_site_3	NotI
cloning_site_5	BamHI
promoter
sequencing_primer_3	BGH-rev
sequencing_primer_5	LNCX
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	HEL-33, HEL-S-72p, HSC54, HSC70, HSC71, HSP71, HSP73, HSPA10, LAP-1, LAP1, NIP71
gene	HSPA8
id	3312

genbank_ids

mutation

name

HSPA8

shRNA_sequence

size

2666

species

9606

Homo sapiens