This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
CMV-NanoLuc-BRAF-CAT
catalog :
194772
citations: 2
Reference
Spencer Smith R, Morrison D. Protocol for measuring BRAF autoinhibition in live cells using a proximity-based NanoBRET assay. STAR Protoc. 2023;4:102461 pubmed publisher
Spencer Smith R, Terrell E, Insinna C, Agamasu C, Wagner M, Ritt D, et al. RASopathy mutations provide functional insight into the BRAF cysteine-rich domain and reveal the importance of autoinhibition in BRAF regulation. Mol Cell. 2022;82:4262-4276.e5 pubmed publisher
product information
Catalog Number :
194772
Product Name :
CMV-NanoLuc-BRAF-CAT
article :
doi10.1016/j.molcel.2022.10.016
id28233370
pubmed_id36347258
bacterial resistance :
Ampicillin
cloning :
backbonepDest-303
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Mammalian expression construct encoding the BRAF catalytic (CAT) domain (AA 435-766) with an N-terminal NanoLuc tag.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BsrGI
cloning_site_5BsrGI
promoterCMV51
sequencing_primer_3
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesB-RAF1, B-raf, BRAF-1, BRAF1, NS7, RAFB1
geneBRAF
id673
genbank_ids
mutation
nameBRAF catalytic (CAT) domain (AA 435-766)
shRNA_sequence
size1000
species
9606
Homo sapiens
tags
locationN terminal on insert
tagNanoLuc
resistance markers :
6274
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA