This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pBABE Gal4(1-94) HSF ABC Flag ac
catalog :
1946
citations: 1
Reference
Sullivan E, Weirich C, Guyon J, Sif S, Kingston R. Transcriptional activation domains of human heat shock factor 1 recruit human SWI/SNF. Mol Cell Biol. 2001;21:5826-37 pubmed
product information
Catalog Number :
1946
Product Name :
pBABE Gal4(1-94) HSF ABC Flag ac
article :
doi10.1128/MCB.21.17.5826-5837.2001
id141
pubmed_id11486022
bacterial resistance :
Ampicillin
cloning :
backbonepBABE-puro
backbone_mutation
backbone_origin
backbone_size5200
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth notes :
contains the FLAG epitope amino terminal to the GAL4 DNA binding domain (amino acids 1 to 94), attached to the C terminus of hHSF1, (amino acids 201 to 529). This construct was subcloned into the pBABE/puro retroviral vector via the BglII and EcoRI sites. Acidic Mutant. (Kingston #1153)
origin :
37
pi :
alt_names
HSF1
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5BamHI/BglII
promoter
sequencing_primer_3
sequencing_primer_5pBABE 5'
site_3_destroyed
site_5_destroyed1
entrez_gene
aliasesHSTF1
geneHSF1
id3297
genbank_ids
mutationacidic mutant (D416, E493, E496 A)
nameHeat Shock Factor 1
shRNA_sequence
size1300
species
9606
Homo sapiens
tags
locationN terminal on insert
tagflag
locationN terminal on insert
tagGal4 DBD
plasmid copy :
Carl Wu
resistance markers :
254
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA