pBABE Gal4 (1-94) HSF ABC Flag wt | Addgene 1945 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pBABE Gal4 (1-94) HSF ABC Flag wt

catalog :

1945

citations: 1

Reference
Sullivan E, Weirich C, Guyon J, Sif S, Kingston R. Transcriptional activation domains of human heat shock factor 1 recruit human SWI/SNF. Mol Cell Biol. 2001;21:5826-37 pubmed

product information

Catalog Number :

1945

Product Name :

pBABE Gal4 (1-94) HSF ABC Flag wt

article :

doi	10.1128/MCB.21.17.5826-5837.2001
id	141
pubmed_id	11486022

bacterial resistance :

Ampicillin

cloning :

backbone

pBABE-puro

backbone_mutation

backbone_origin

backbone_size

5200

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Retroviral

growth notes :

contains the FLAG epitope amino terminal to the GAL4 DNA binding domain (amino acids 1 to 94), attached to the C terminus of hHSF1, (amino acids 201 to 529). This construct was subcloned into the pBABE/puro retroviral vector via the BglII and EcoRI sites. (Kingston #1152)

origin :

pi :

alt_names

HSF1

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRI
cloning_site_5	BamHI/BglII
promoter
sequencing_primer_3
sequencing_primer_5	pBABE 5'
site_3_destroyed
site_5_destroyed	1

entrez_gene

aliases	HSTF1
gene	HSF1
id	3297

genbank_ids

mutation

name

Heat Shock Factor 1

shRNA_sequence

size

1300

species

9606

Homo sapiens