This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Punc-4c.TIR1
catalog :
194053
citations: 1
Reference
Kurashina M, Mizumoto K. Targeting endogenous proteins for spatial and temporal knockdown using auxin-inducible degron in Caenorhabditis elegans. STAR Protoc. 2023;4:102028 pubmed publisher
product information
Catalog Number :
194053
Product Name :
Punc-4c.TIR1
article :
doi10.1016/j.xpro.2022.102028
id28233232
pubmed_id36640369
bacterial resistance :
Ampicillin
cloning :
backbonedelta-pSM
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Worm Expression
growth strain :
TIR1 expression vector under the unc-4c promoter expressed in the DA motor neurons in C. elegans
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3KpnI
cloning_site_5AscI
promoterunc-4c
sequencing_primer_3unc-54 utr R cttagaagtcagaggcacgg
sequencing_primer_5unc-4c F GAAGTCTAGCAGGATTAAGC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAT3G62980, AtTIR1, TRANSPORT INHIBITOR RESPONSE 1
geneTIR1
id825473
genbank_ids
mutationcodon-optimized for expression in C. elegans; Two point mutations D170E and M473L incorporated; Derived from pLZ31 (Addgene Plasmid #71720)
nameTIR1
shRNA_sequence
size1887
species
3702
Arabidopsis thaliana
tags
plasmid copy :
The TIR1 sequence was PCR-amplified from pLZ31 (pCFJ151_Peft-3_TIR1_linker_mRuby_unc-54 3'UTR) (Plasmid #71720).
resistance markers :
4144
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA