This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
PH-miRFP-FRBx2
catalog :
193569
citations: 1
Reference
Obashi K, Sochacki K, Strub M, Taraska J. A conformational switch in clathrin light chain regulates lattice structure and endocytosis at the plasma membrane of mammalian cells. Nat Commun. 2023;14:732 pubmed publisher
product information
Catalog Number :
193569
Product Name :
PH-miRFP-FRBx2
article :
doi10.1038/s41467-023-36304-7
id28233154
pubmed_id36759616
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-C1
backbone_mutation
backbone_originClontech
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Please visit https://doi.org/10.1101/2022.03.21.485138 for bioRxiv preprint.
growth strain :
Expresses PH domain from PLC delta1 tagged with miRFP703 and two FRB mutants (T2098L) in mammalian cells.
origin :
37
pi :
alt_names
Plcd1
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3SV40pArev
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesPlc1
genePlcd1
id24655
genbank_ids
mutationPH domain
namePH domain from PLC delta1
shRNA_sequence
size407
species
10116
Rattus norvegicus
tags
locationC terminal on insert
tagmiRFP703
locationC terminal on insert
tagFRB (T2098L)
locationC terminal on insert
tagFRB (T2098L)
plasmid copy :
Dr. T. Inoue (Johns Hopkins University) and addgene #79997
resistance markers :
2782
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA