This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
T7-VEE-CFP-e2a-SOX2-17-p2a-KLF4
catalog :
193360
product information
Catalog Number :
193360
Product Name :
T7-VEE-CFP-e2a-SOX2-17-p2a-KLF4
article :
doi10.1016/j.stem.2023.11.010
id28243845
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backboneT7-VEE-IRES-puro (Addgene plasmid 58970)
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Other
Venezuelan equine encephalitis (VEE) virus RNA replicon
growth notes :
Please visit https://www.biorxiv.org/content/10.1101/2022.09.23.509242v1 for BioRxiv preprint
growth strain :
VEE-CFP-S*K (self-replicating RNA vector expressing CFP + human super-SOX + KLF4 + PuroR) for integration-free na ve conversion
origin :
37
pi :
alt_names
Cyan Fluorescent Protein
S*
superSOX
cloning
clone_methodRestriction Enzyme
cloning_site_3ClaI
cloning_site_5ClaI
promoterT7
sequencing_primer_3TATAGACAAACGCACACCG
sequencing_primer_5CGGCTAACCTGAATGGACTACGAC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesEZF, GKLF
geneKLF4
id9314
aliasesANOP3, MCOPS3
geneSOX2
id6657
genbank_ids
mutationSOX2-17 is engineered highly cooperative chimeric transcription factor, where the following elements of SOX2 were swapped with SOX17: 43-47, 61, 65-86 and CTD
nameCFP-e2a-SOX2-17-p2a-KLF4
shRNA_sequence
size3477
species
9606
Homo sapiens
32644
Other
Aequorea victoria
tags
resistance markers :
769
tags :
Low Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA