Flag pLJM1 Rheb1 | Addgene 19312 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

Flag pLJM1 Rheb1

catalog :

19312

citations: 3

Reference
Norambuena A, Wallrabe H, Cao R, Wang D, Silva A, Švindrych Z, et al. A novel lysosome-to-mitochondria signaling pathway disrupted by amyloid-β oligomers. EMBO J. 2018;37: pubmed publisher
Norambuena A, Wallrabe H, McMahon L, Silva A, Swanson E, Khan S, et al. mTOR and neuronal cell cycle reentry: How impaired brain insulin signaling promotes Alzheimer's disease. Alzheimers Dement. 2017;13:152-167 pubmed publisher
Sancak Y, Peterson T, Shaul Y, Lindquist R, Thoreen C, Bar Peled L, et al. The Rag GTPases bind raptor and mediate amino acid signaling to mTORC1. Science. 2008;320:1496-501 pubmed publisher

product information

Catalog Number :

19312

Product Name :

Flag pLJM1 Rheb1

article :

doi	10.1126/science.1157535
id	2268
pubmed_id	18497260

bacterial resistance :

Ampicillin

cloning :

backbone

pLJM1

backbone_mutation

backbone_origin

backbone_size

7400

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

growth notes :

pLJM1 is based on pLKO.1. pLJM1 was cut with AgeI and EcoRI and a Flag tag was cloned in. The cDNA was cloned into Flag-pLJM1 with SalI and EcoRV. The sequence across the MCS includes AgeI-Flag-SalI-gene-EcoRV-EcoRI

growth temp :

Grow in Exel10Gold or Stbl2 cells

origin :

pi :

alt_names

RHEB

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRV
cloning_site_5	SalI
promoter
sequencing_primer_3
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	RHEB2
gene	RHEB
id	6009

genbank_ids

mutation

name

RHEB Ras homolog enriched in brain

shRNA_sequence

size

560

species

9606

Homo sapiens