This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pRK5-HA GST Rheb1
catalog :
19310
citations: 5
Reference
Cheah P, Prabhakar S, Yellen D, Beauchamp R, Zhang X, Kasamatsu S, et al. Gene therapy for tuberous sclerosis complex type 2 in a mouse model by delivery of AAV9 encoding a condensed form of tuberin. Sci Adv. 2021;7: pubmed publisher
Shibutani S, Okazaki H, Iwata H. Dynamin-dependent amino acid endocytosis activates mechanistic target of rapamycin complex 1 (mTORC1). J Biol Chem. 2017;292:18052-18061 pubmed publisher
Rui Y, Xu Z, Chen Z, Zhang S. The GST-BHMT assay reveals a distinct mechanism underlying proteasome inhibition-induced macroautophagy in mammalian cells. Autophagy. 2015;11:812-32 pubmed publisher
Musiwaro P, Smith M, Manifava M, Walker S, Ktistakis N. Characteristics and requirements of basal autophagy in HEK 293 cells. Autophagy. 2013;9:1407-17 pubmed publisher
Sancak Y, Peterson T, Shaul Y, Lindquist R, Thoreen C, Bar Peled L, et al. The Rag GTPases bind raptor and mediate amino acid signaling to mTORC1. Science. 2008;320:1496-501 pubmed publisher
product information
Catalog Number :
19310
Product Name :
pRK5-HA GST Rheb1
article :
doi10.1126/science.1157535
id2268
pubmed_id18497260
bacterial resistance :
Ampicillin
cloning :
backbonepRK5
backbone_mutation
backbone_originBD PharMingen
backbone_size4754
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
origin :
37
pi :
alt_names
RHEB
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5SalI
promoter
sequencing_primer_3pBABE 3'
sequencing_primer_5SP6, pGEX 5'
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesRHEB2
geneRHEB
id6009
genbank_ids
mutation
nameRHEB Ras homolog enriched in brain
shRNA_sequence
size560
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagHA
locationN terminal on backbone
tagGST
resistance markers :
432
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA