This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pRK5-HA GST RagC 75L
catalog :
19305
citations: 17
Reference
Malik N, Ferreira B, Hollstein P, Curtis S, Trefts E, Weiser Novak S, et al. Induction of lysosomal and mitochondrial biogenesis by AMPK phosphorylation of FNIP1. Science. 2023;380:eabj5559 pubmed publisher
Curnock R, Yalci K, Palmfeldt J, J xe4 xe4 ttel xe4 M, Liu B, Carroll B. TFEB-dependent lysosome biogenesis is required for senescence. EMBO J. 2023;42:e111241 pubmed publisher
Nakamura J, Yamamoto T, Takabatake Y, Namba Hamano T, Minami S, Takahashi A, et al. TFEB-mediated lysosomal exocytosis alleviates high-fat diet-induced lipotoxicity in the kidney. JCI Insight. 2023;8: pubmed publisher
Akwa Y, Di Malta C, Zallo F, Gondard E, Lunati A, Diaz de Grenu L, et al. Stimulation of synaptic activity promotes TFEB-mediated clearance of pathological MAPT/Tau in cellular and mouse models of tauopathies. Autophagy. 2023;19:660-677 pubmed publisher
Mohanasundaram P, Coelho Rato L, Modi M, Urbanska M, Lautenschl xe4 ger F, Cheng F, et al. Cytoskeletal vimentin regulates cell size and autophagy through mTORC1 signaling. PLoS Biol. 2022;20:e3001737 pubmed publisher
Du X, Di Malta C, Fang Z, Shen T, Niu X, Chen M, et al. Nuciferine protects against high-fat diet-induced hepatic steatosis and insulin resistance via activating TFEB-mediated autophagy-lysosomal pathway. Acta Pharm Sin B. 2022;12:2869-2886 pubmed publisher
Wrobel M, Cendrowski J, Szymańska E, Grębowicz Maciukiewicz M, Budick Harmelin N, Macias M, et al. ESCRT-I fuels lysosomal degradation to restrict TFEB/TFE3 signaling via the Rag-mTORC1 pathway. Life Sci Alliance. 2022;5: pubmed publisher
Alesi N, Akl E, Khabibullin D, Liu H, Nidhiry A, Garner E, et al. TSC2 regulates lysosome biogenesis via a non-canonical RAGC and TFEB-dependent mechanism. Nat Commun. 2021;12:4245 pubmed publisher
Hirst J, Hesketh G, Gingras A, Robinson M. Rag GTPases and phosphatidylinositol 3-phosphate mediate recruitment of the AP-5/SPG11/SPG15 complex. J Cell Biol. 2021;220: pubmed publisher
Demian W, Persaud A, Jiang C, Coyaud E, Liu S, Kapus A, et al. The Ion Transporter NKCC1 Links Cell Volume to Cell Mass Regulation by Suppressing mTORC1. Cell Rep. 2019;27:1886-1896.e6 pubmed publisher
Pal R, Palmieri M, Chaudhury A, Klisch T, di Ronza A, Neilson J, et al. Src regulates amino acid-mediated mTORC1 activation by disrupting GATOR1-Rag GTPase interaction. Nat Commun. 2018;9:4351 pubmed publisher
Jia J, Abudu Y, Claude Taupin A, Gu Y, Kumar S, Choi S, et al. Galectins Control mTOR in Response to Endomembrane Damage. Mol Cell. 2018;70:120-135.e8 pubmed publisher
Khayati K, Antikainen H, Bonder E, Weber G, Kruger W, Jakubowski H, et al. The amino acid metabolite homocysteine activates mTORC1 to inhibit autophagy and form abnormal proteins in human neurons and mice. FASEB J. 2017;31:598-609 pubmed publisher
Li R, Xu J, Fu C, Zhang J, Zheng Y, Jia H, et al. Regulation of mTORC1 by lysosomal calcium and calmodulin. elife. 2016;5: pubmed publisher
Durán R, MacKenzie E, Boulahbel H, Frezza C, Heiserich L, Tardito S, et al. HIF-independent role of prolyl hydroxylases in the cellular response to amino acids. Oncogene. 2013;32:4549-56 pubmed publisher
Cully M, Genevet A, Warne P, Treins C, Liu T, Bastien J, et al. A role for p38 stress-activated protein kinase in regulation of cell growth via TORC1. Mol Cell Biol. 2010;30:481-95 pubmed publisher
Sancak Y, Peterson T, Shaul Y, Lindquist R, Thoreen C, Bar Peled L, et al. The Rag GTPases bind raptor and mediate amino acid signaling to mTORC1. Science. 2008;320:1496-501 pubmed publisher
product information
Catalog Number :
19305
Product Name :
pRK5-HA GST RagC 75L
article :
doi10.1126/science.1157535
id2268
pubmed_id18497260
bacterial resistance :
Ampicillin
cloning :
backbonepRK5
backbone_mutation
backbone_originBD PharMingen
backbone_size4754
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
origin :
37
pi :
alt_names
RRAGC
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5SalI
promoter
sequencing_primer_3pBABE 3'
sequencing_primer_5SP6, pGEX 5'
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesGTR2, RAGC, TIB929
geneRRAGC
id64121
genbank_ids
mutationS75L
nameRas-related GTP binding C
shRNA_sequence
size1426
species
9606
Homo sapiens
tags
locationN terminal on insert
tagHA
locationN terminal on insert
tagGST
resistance markers :
432
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA