pPGS dnTcf-4(deltaN41) | Addgene 19284 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pPGS dnTcf-4(deltaN41)

catalog :

19284

citations: 5

Reference
Moreno Londo xf1 o A, Casta xf1 eda Patl xe1 n M, Sarabia S xe1 nchez M, Mac xed as Silva M, Robles Flores M. Canonical Wnt Pathway Is Involved in Chemoresistance and Cell Cycle Arrest Induction in Colon Cancer Cell Line Spheroids. Int J Mol Sci. 2023;24: pubmed publisher
Rappaport J, Entezari A, Caspi A, Caksa S, Jhaveri A, Stanek T, et al. A β-Catenin-TCF-Sensitive Locus Control Region Mediates GUCY2C Ligand Loss in Colorectal Cancer. Cell Mol Gastroenterol Hepatol. 2021;: pubmed publisher
Jian Y, Wang M, Zhang Y, Ou R, Zhu Z, Ou Y, et al. Jade family PHD finger 3 (JADE3) increases cancer stem cell-like properties and tumorigenicity in colon cancer. Cancer Lett. 2018;428:1-11 pubmed publisher
Pai P, Rachagani S, Lakshmanan I, Macha M, Sheinin Y, Smith L, et al. The canonical Wnt pathway regulates the metastasis-promoting mucin MUC4 in pancreatic ductal adenocarcinoma. Mol Oncol. 2016;10:224-39 pubmed publisher
Kolligs F, Hu G, Dang C, Fearon E. Neoplastic transformation of RK3E by mutant beta-catenin requires deregulation of Tcf/Lef transcription but not activation of c-myc expression. Mol Cell Biol. 1999;19:5696-706 pubmed

product information

Catalog Number :

19284

Product Name :

pPGS dnTcf-4(deltaN41)

article :

doi
id	2057
pubmed_id	10409758

bacterial resistance :

Ampicillin

cloning :

backbone

pPGS-CMV-CITE-Neo

backbone_mutation

backbone_origin

G. Nabel (U. of Michigan)

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Retroviral

growth notes :

The pPGS-CMV-CITE-neo vector allows the expression of chimeric transcripts encoding a gene of interest fused to the neomycin resistance gene. Expression of the neomycin resistance protein results from an internal ribosome entry site upstream of the neomycin open reading frame.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRI
cloning_site_5	NotI
promoter
sequencing_primer_3	IRES-R
sequencing_primer_5	T3
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CDG2T, E2-2, FCD2, FECD3, ITF-2, ITF2, PTHS, SEF-2, SEF2, SEF2-1, SEF2-1A, SEF2-1B, SEF2-1D, TCF-4, bHLHb19
gene	TCF4
id	6925

genbank_ids

mutation

Dominant negative, lacks N-terminal 41 amino acids

name

Tcf-4

shRNA_sequence

size

species

9606

Homo sapiens