This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pBS-GBX2-CDX124-Ef1a-mCherry-Neo
catalog :
192823
citations: 1
Reference
Maimaitili M, Chen M, Febbraro F, Ucuncu E, Kelly R, Niclis J, et al. Enhanced production of mesencephalic dopaminergic neurons from lineage-restricted human undifferentiated stem cells. Nat Commun. 2023;14:7871 pubmed publisher
product information
Catalog Number :
192823
Product Name :
pBS-GBX2-CDX124-Ef1a-mCherry-Neo
article :
doi10.1038/s41467-023-43471-0
id28229600
pubmed_id38052784
bacterial resistance :
Ampicillin
cloning :
backbonepBS-BsmbI-LoxP-Ef1a-EM7-mCherry-Neo-PolyA-LoxP
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Cre/Lox
CRISPR
growth notes :
CDX1 gRNA sequence: CTACACCGACCACCAACGCC CDX2 gRNA sequence: GTACACGGACCACCAGCGGC CDX4 gRNA sequence: CCTGGGCCTTTCCGAGAGAC GBX2 gRNA sequence: AAAGGTGGAAGACGACCCGA
growth strain :
Expresses mCherry and four sgRNAs: GBX2, CDX1, CDX2, CDX4
growth temp :
plasmid is also kanamycin resistant
origin :
37
pi :
alt_names
cloning
entrez_gene
aliasesCDX-3, CDX2/AS, CDX3
geneCDX2
id1045
aliases
geneCDX1
id1044
aliases
geneCDX4
id1046
aliases
geneGBX2
id2637
genbank_ids
mutation
namemCherry and sgRNA CDX1 CDX2 CDX4 and GBX2
shRNA_sequence
size
species
100
Synthetic
tags
resistance markers :
4193
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA