This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pFastBac dRING1F
catalog :
1928
citations: 1
Reference
Francis N, Saurin A, Shao Z, Kingston R. Reconstitution of a functional core polycomb repressive complex. Mol Cell. 2001;8:545-56 pubmed
product information
Catalog Number :
1928
Product Name :
pFastBac dRING1F
article :
doi10.1016/S1097-2765(01)00316-1
id136
pubmed_id11583617
bacterial resistance :
Ampicillin
cloning :
backbonepFastBac
backbone_mutation
backbone_originInvitrogen
backbone_size4800
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Insect Expression
growth notes :
For baculoviral expression of dRING1F with C-terminal flag epitope. 300bp of C-terminus of pFB-dRING1 was amplified with SacI--FLAG-KpnI primers and cloned into dRING1 with SacI--KpnI to produce dRING1 with C-terminal FLAG epitope (kingston #1325)
origin :
37
pi :
alt_names
Sce
cloning
clone_methodRestriction Enzyme
cloning_site_3KpnI
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5Polyhedrin forward
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDmel_CG5595, CG5595, DRING, Ding, Dmel CG5595, Dring, RING, RING1, Ring, Ring/Sce, Rnf2, SCE, Sce/dRing, dRING, dRING1, dRing, dRing1, dring, sce
geneSce
id43327
genbank_ids
mutation
namedRING1
shRNA_sequence
size1500
species
7227
Drosophila melanogaster
tags
locationC terminal on insert
tagflag
resistance markers :
254
tags :
High Copy
terms :
Gentamicin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA