This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3-mCherry-TSC2(AA)-NLS
catalog :
192443
citations: 1
Reference
Zhong Y, Zhou X, Guan K, Zhang J. Rheb regulates nuclear mTORC1 activity independent of farnesylation. Cell Chem Biol. 2022;29:1037-1045.e4 pubmed publisher
product information
Catalog Number :
192443
Product Name :
pcDNA3-mCherry-TSC2(AA)-NLS
article :
doi10.1016/j.chembiol.2022.02.006
id28229612
pubmed_id35294906
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3
backbone_mutation
backbone_origin
backbone_size5472
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A mutations to block Akt phosphorylation; targeted to the nucleus.
origin :
37
pi :
alt_names
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3BGH reverse
sequencing_primer_5CMV forward
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesLAM, PPP1R160, TSC4
geneTSC2
id7249
genbank_ids
mutationTSC2 Ser 939 and Thr 1439 (equivalent to 1462 in isoform 1) mutated to Ala.
namemCherry-TSC2(S939A,T1439A)-NLS
shRNA_sequence
size6117
species
9606
Homo sapiens
tags
locationN terminal on backbone
tag6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag
locationN terminal on insert
tagmCherry
locationC terminal on insert
tagNuclear localization signal (NLS)
plasmid copy :
TSC2-AA cDNA was provided by Brendan Manning (Addgene Plasmid #14131)
resistance markers :
2182
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA