This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3-Lyso-mCherry-Rheb(C181S)
catalog :
192438
citations: 1
Reference
Zhong Y, Zhou X, Guan K, Zhang J. Rheb regulates nuclear mTORC1 activity independent of farnesylation. Cell Chem Biol. 2022;29:1037-1045.e4 pubmed publisher
product information
Catalog Number :
192438
Product Name :
pcDNA3-Lyso-mCherry-Rheb(C181S)
article :
doi10.1016/j.chembiol.2022.02.006
id28229612
pubmed_id35294906
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3
backbone_mutation
backbone_origin
backbone_size5415
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Encodes mCherry-tagged, farnesylation-deficient Rheb C181S mutant targeted to lysosome membrane.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5HindIII
promoterCMV
sequencing_primer_3BGH reverse
sequencing_primer_5CMV forward
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesRHEB2
geneRHEB
id6009
genbank_ids
mutationRheb Cys 181 mutated to Ser; abolishes C-terminal farnesylation.
nameLyso-mCherry-Rheb(C181S)
shRNA_sequence
size2547
species
9606
Homo sapiens
tags
locationN terminal on insert
tagFull-length LAMP1
locationN terminal on insert
tagmCherry
resistance markers :
2182
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA