This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pDEST_6xUAS:mNeonGreen-Rab5a
catalog :
192355
citations: 1
Reference
M xf6 ller K, Brambach M, Villani A, Gallo E, Gilmour D, Peri F. A role for the centrosome in regulating the rate of neuronal efferocytosis by microglia in vivo. elife. 2022;11: pubmed publisher
product information
Catalog Number :
192355
Product Name :
pDEST_6xUAS:mNeonGreen-Rab5a
article :
doi
id28229606
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backboneGateway Tol2 destination vector with cmlc2:mRFP-SV40pA for zebrafish red heart transgenesis marker
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Please visit for https://www.biorxiv.org/content/10.1101/2022.08.12.503784v1 bioRxiv preprint.
growth strain :
zfRabaa tagged with mNeonGreen, expressed under the UAS promoter
origin :
37
pi :
alt_names
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3CCACATTTGTAGAGGTTTTACTTGC
sequencing_primer_5gtctgaaacacaggccagat
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesfj38g08, hm:zehn1144, rab5a, wu:fj38g08
generab5aa
id337737
genbank_ids
337737
mutation
nameRab5a
shRNA_sequence
size651
species
7955
Danio rerio
tags
locationN terminal on insert
tagmNeonGreen
plasmid copy :
The Rab5aa cDNA was a gift from the Gilmour lab, originally published in Hartmann et al., 2020.
resistance markers :
6190
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA