This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCMV-PARP1_R878A-mCherry
catalog :
192261
citations: 1
Reference
Arnold M, Langelier M, Gartrell J, Kirby I, Sanderson D, Bejan D, et al. Allosteric regulation of DNA binding and target residence time drive the cytotoxicity of phthalazinone-based PARP-1 inhibitors. Cell Chem Biol. 2022;29:1694-1708.e10 pubmed publisher
product information
Catalog Number :
192261
Product Name :
pCMV-PARP1_R878A-mCherry
article :
doi10.1016/j.chembiol.2022.11.006
id28229596
pubmed_id36493759
bacterial resistance :
Kanamycin
cloning :
backbonepCMV-PARP1-mCherry
backbone_mutation
backbone_origin
backbone_size7154
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Expresses PARP1 R878A mutant in mammalian cells. Tagged with mCherry.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3AgeI
cloning_site_5EcoRV
promoterCMV
sequencing_primer_3SV40
sequencing_primer_5CMV
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesADPRT, ADPRT 1, ADPRT1, ARTD1, PARP, PARP-1, PARS, PPOL, Poly-PARP, pADPRT-1
genePARP1
id142
genbank_ids
mutationR878A
namePARP1_R878A
shRNA_sequence
size557
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagmCherry
plasmid copy :
Backbone gifted from Dr. G. Timinszky (Hungarian Academy of Sciences)
resistance markers :
6030
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA