This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLenti_CMVTRE3G_LYSET-Isoform2_Flag
catalog :
191999
citations: 1
Reference
Richards C, Jabs S, Qiao W, Varanese L, Schweizer M, Mosen P, et al. The human disease gene LYSET is essential for lysosomal enzyme transport and viral infection. Science. 2022;378:eabn5648 pubmed publisher
product information
Catalog Number :
191999
Product Name :
pLenti_CMVTRE3G_LYSET-Isoform2_Flag
article :
doi10.1126/science.abn5648
id28229554
pubmed_id36074821
bacterial resistance :
Ampicillin
cloning :
backbonepLenti CMVTRE3G Puro DEST (w811-1)
backbone_mutation
backbone_originaddgene #27565
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth strain :
Lentiviral vector to generate flag-tagged LYSET(TMEM251)-isoform2 stable expressing cell line under CMVTRE3G promoter
origin :
30
pi :
alt_names
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterCMVTRE3G
sequencing_primer_3CATTAAAGCAGCGTATCCACATAGC
sequencing_primer_5GAACCGTCAGATCGCCTGG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesC14orf109, DMAN, GCAF, TMEM251
geneLYSET
id26175
genbank_ids
mutation
nameLYSET-Isoform2
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationC terminal on insert
tagFlag
resistance markers :
5351
tags :
Unknown
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA