This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pBabe Angptl4 puro
catalog :
19156
citations: 2
Reference
Li L, Che L, Tharp K, Park H, Pilo M, Cao D, et al. Differential requirement for de novo lipogenesis in cholangiocarcinoma and hepatocellular carcinoma of mice and humans. Hepatology. 2016;63:1900-13 pubmed publisher
Padua D, Zhang X, Wang Q, Nadal C, Gerald W, Gomis R, et al. TGFbeta primes breast tumors for lung metastasis seeding through angiopoietin-like 4. Cell. 2008;133:66-77 pubmed publisher
product information
Catalog Number :
19156
Product Name :
pBabe Angptl4 puro
article :
doi10.1016/j.cell.2008.01.046
id1925
pubmed_id18394990
bacterial resistance :
Ampicillin
cloning :
backbonepBABE-puro
backbone_mutation
backbone_origin
backbone_size5169
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth notes :
PCR out Angptl4 cDNA, used TOPO cloning kit and generated BglII sites at the ends. Cloned into BamHI site of pBabe which destroys BamHI site. Checked orientation with EcoRI digest.
growth temp :
Need to grow in STBL3
origin :
37
pi :
alt_names
ANGPTL4, ANGPTL2, ARP4, FIAF, HFARP, NL2, PGAR, pp1158
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5BamHI
promoter
sequencing_primer_3pBABE 3'
sequencing_primer_5pBABE 5'
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesARP4, FIAF, HARP, HFARP, NL2, PGAR, TGQTL, UNQ171, pp1158
geneANGPTL4
id51129
genbank_ids
mutation
nameangiopoietin-like 4
shRNA_sequence
size1200
species
9606
Homo sapiens
tags
plasmid copy :
David Padua
resistance markers :
313
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA