This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pRetroSuper shSmad4J puro
catalog :
19152
citations: 1
Reference
Padua D, Zhang X, Wang Q, Nadal C, Gerald W, Gomis R, et al. TGFbeta primes breast tumors for lung metastasis seeding through angiopoietin-like 4. Cell. 2008;133:66-77 pubmed publisher
product information
Catalog Number :
19152
Product Name :
pRetroSuper shSmad4J puro
article :
doi10.1016/j.cell.2008.01.046
id1925
pubmed_id18394990
bacterial resistance :
Ampicillin
cloning :
backbonepRetroSuper-Puro
backbone_mutation
backbone_origin
backbone_size6400
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
RNAi
growth notes :
19nt targeting sequence - 5'-GGTGTGCAGTTGGAATGTA- 3' (SMAD4)
origin :
37
pi :
alt_names
DPC4, JIP, MADH4
cloning
clone_methodRestriction Enzyme
cloning_site_3HindIII
cloning_site_5BglII
promoter
sequencing_primer_3
sequencing_primer_5pBABE 5'
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDPC4, JIP, MADH4, MYHRS
geneSMAD4
id4089
genbank_ids
mutation
namesmall hairpin RNA against SMAD family member 4
shRNA_sequence
size64
species
9606
Homo sapiens
tags
plasmid copy :
David Padua
resistance markers :
313
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA