This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCDH-EFS-FlpO
catalog :
191509
citations: 1
Reference
Orstad G, Fort G, Parnell T, Jones A, Stubben C, Lohman B, et al. FoxA1 and FoxA2 control growth and cellular identity in NKX2-1-positive lung adenocarcinoma. Dev Cell. 2022;57:1866-1882.e10 pubmed publisher
product information
Catalog Number :
191509
Product Name :
pCDH-EFS-FlpO
article :
doi10.1016/j.devcel.2022.06.017
id28229489
pubmed_id35835117
bacterial resistance :
Ampicillin
cloning :
backbonepCDH
backbone_mutationEFS promoter driving FlpO expression
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth strain :
Lentiviral Flp Recombinase driven by the EFS promoter
origin :
37
pi :
alt_names
EFS promoter driving FlpO recombinase
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5SnaI
promoterEF1a/EFS promoter inserted
sequencing_primer_3GATGTCGAACTGGCTCAT
sequencing_primer_5ccagtacatgaccttatggg
site_3_destroyed
site_5_destroyed1
entrez_gene
aliases
geneflp
id850474
genbank_ids
mutation
nameflp recombinase
shRNA_sequence
size212
species
tags
plasmid copy :
We replaced the CMV promoter from pCDH-CMV-FlpO (Camolotto et al 2018) with an EF1a/EFS promoter from the pCDH-Cre plasmid (Han et al 2014)
resistance markers :
6161
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA