This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
CD44S pWZL-Blast
catalog :
19126
citations: 2
Reference
Yun E, Zhou J, Lin C, Hernandez E, Fazli L, Gleave M, et al. Targeting Cancer Stem Cells in Castration-Resistant Prostate Cancer. Clin Cancer Res. 2016;22:670-9 pubmed publisher
Godar S, Ince T, Bell G, Feldser D, Donaher J, Bergh J, et al. Growth-inhibitory and tumor- suppressive functions of p53 depend on its repression of CD44 expression. Cell. 2008;134:62-73 pubmed publisher
product information
Catalog Number :
19126
Product Name :
CD44S pWZL-Blast
article :
doi10.1016/j.cell.2008.06.006
id2244
pubmed_id18614011
bacterial resistance :
Ampicillin
cloning :
backbonepWZL Blast
backbone_mutation
backbone_originMillenium Pharmaceuticals
backbone_size5100
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth notes :
Human CD44 cDNA,standard form no variable exons. CD44 protein is stably expressed in MCF7Ras or HMEC-T cells but only transiently in BPEC-T cells. Use pBabe-CD44S for BPEC-T cells.
growth temp :
Use Stbl3 or HB101 to reduce chance of recombination.
origin :
37
pi :
alt_names
CDW44, CSPG8, ECMR-III, HCELL, IN, LHR, MC56, MDU2, MDU3, MGC10468, MIC4, MUTCH-I, Pgp1
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5XhoI
promoter
sequencing_primer_3
sequencing_primer_5pBABE 5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCDW44, CSPG8, ECM-III, ECMR-III, H-CAM, HCELL, HUTCH-1, HUTCH-I, Hermes-1, IN, LHR, MC56, MDU2, MDU3, MIC4, Pgp1
geneCD44
id960
genbank_ids
mutation
nameCD44 standard
shRNA_sequence
size1300
species
9606
Homo sapiens
tags
resistance markers :
568
tags :
High Copy
terms :
Blasticidin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA