shCD44-2 pRRL | Addgene 19123 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

shCD44-2 pRRL

catalog :

19123

citations: 4

Reference
Spertini C, Baïsse B, Bellone M, Gikic M, Smirnova T, Spertini O. Acute Myeloid and Lymphoblastic Leukemia Cell Interactions with Endothelial Selectins: Critical Role of PSGL-1, CD44 and CD43. Cancers (Basel). 2019;11: pubmed publisher
Weitzenfeld P, Meshel T, Ben Baruch A. Microenvironmental networks promote tumor heterogeneity and enrich for metastatic cancer stem-like cells in Luminal-A breast tumor cells. Oncotarget. 2016;7:81123-81143 pubmed publisher
Zhao S, Chen C, Chang K, Karnad A, Jagirdar J, Kumar A, et al. CD44 Expression Level and Isoform Contributes to Pancreatic Cancer Cell Plasticity, Invasiveness, and Response to Therapy. Clin Cancer Res. 2016;22:5592-5604 pubmed publisher
Godar S, Ince T, Bell G, Feldser D, Donaher J, Bergh J, et al. Growth-inhibitory and tumor- suppressive functions of p53 depend on its repression of CD44 expression. Cell. 2008;134:62-73 pubmed publisher

product information

Catalog Number :

19123

Product Name :

shCD44-2 pRRL

article :

doi	10.1016/j.cell.2008.06.006
id	2244
pubmed_id	18614011

bacterial resistance :

Ampicillin

cloning :

backbone

pRRL-U6-siluc-cppt-hPGKGFPSIN

backbone_mutation

backbone_origin

backbone_size

7247

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

growth notes :

Please note that the shRNA oligo present in this plasmid is referred to as shCD44-3 in the associated publication.
sense:5'-CCGGTATGCAATGTGCTACTGATTGTCTCG
AGACAATCAGTAGCACATTGCATTTTTTG-3'
shRNA to human CD44 (should target all isoforms). Subcloned via NdeI-BamHI frm pLKO1.Puro. This insert replaced identical sequence present in pRRL-EGFP and added the shRNA sequence shRNA oligo sequence:

growth temp :

Use Stbl3 or HB101 to reduce chance of recombination. Grow at 37C, grows slowly.

origin :

pi :

alt_names

CDW44, CSPG8, ECMR-III, HCELL, IN, LHR, MC56, MDU2, MDU3, MGC10468, MIC4, MUTCH-I, Pgp1

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRI
cloning_site_5	AgeI
promoter	U6
sequencing_primer_3	EGFP-N
sequencing_primer_5	U6-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CDW44, CSPG8, ECM-III, ECMR-III, H-CAM, HCELL, HUTCH-1, HUTCH-I, Hermes-1, IN, LHR, MC56, MDU2, MDU3, MIC4, Pgp1
gene	CD44
id	960

genbank_ids

mutation

name

small hairpin RNA against CD44

shRNA_sequence

size

species

9606

Homo sapiens