This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
shp53 pLKO.1 puro
catalog :
19119
citations: 35
Reference
Wang P, Liu X, Chen Y, Jun Hao E, Yao Z, Min Wen J, et al. Adult progenitor rejuvenation with embryonic factors. Cell Prolif. 2023;56:e13459 pubmed publisher
Ghosh M, Saha S, Li J, Montrose D, Martinez L. p53 engages the cGAS/STING cytosolic DNA sensing pathway for tumor suppression. Mol Cell. 2023;83:266-280.e6 pubmed publisher
Alalem M, Bhosale M, Ranjan A, Yamamoto S, Kaida A, Nishikawa S, et al. Mutant p53 Depletion by Novel Inhibitors for HSP40/J-Domain Proteins Derived from the Natural Compound Plumbagin. Cancers (Basel). 2022;14: pubmed publisher
Lama R, Xu C, Galster S, Querol Garc xed a J, Portwood S, Mavis C, et al. Small molecule MMRi62 targets MDM4 for degradation and induces leukemic cell apoptosis regardless of p53 status. Front Oncol. 2022;12:933446 pubmed publisher
Dale K, Armond J, Hynds R, Vladimirou E. Modest increase of KIF11 expression exposes fragilities in the mitotic spindle, causing chromosomal instability. J Cell Sci. 2022;135: pubmed publisher
Li J, Lama R, Galster S, Inigo J, Wu J, Chandra D, et al. Small-Molecule MMRi62 Induces Ferroptosis and Inhibits Metastasis in Pancreatic Cancer via Degradation of Ferritin Heavy Chain and Mutant p53. Mol Cancer Ther. 2022;21:535-545 pubmed publisher
Gandhi N, Oturkar C, Das G. Estrogen Receptor-Alpha and p53 Status as Regulators of AMPK and mTOR in Luminal Breast Cancer. Cancers (Basel). 2021;13: pubmed publisher
Xia Y, Chen S, Cui J, Wang Y, Liu X, Shen Y, et al. Berberine suppresses bladder cancer cell proliferation by inhibiting JAK1-STAT3 signaling via upregulation of miR-17-5p. Biochem Pharmacol. 2021;188:114575 pubmed publisher
Ghosh M, Saha S, Bettke J, Nagar R, Parrales A, Iwakuma T, et al. Mutant p53 suppresses innate immune signaling to promote tumorigenesis. Cancer Cell. 2021;: pubmed publisher
Zheng C, Hu Y, Sakurai M, Pinzon Arteaga C, Li J, Wei Y, et al. Cell Competition Constitutes a Barrier for Interspecies Chimerism. Nature. 2021;: pubmed publisher
Cai J, Xia J, Zou J, Wang Q, Ma Q, Sun R, et al. The PI3K/mTOR dual inhibitor NVP-BEZ235 stimulates mutant p53 degradation to exert anti-tumor effects on triple-negative breast cancer cells. FEBS Open Bio. 2020;10:535-545 pubmed publisher
Shihabudeen Haider Ali M, Cheng X, Moran M, Haemmig S, Naldrett M, Alvarez S, et al. LncRNA Meg3 protects endothelial function by regulating the DNA damage response. Nucleic Acids Res. 2019;47:1505-1522 pubmed publisher
Bakht M, Derecichei I, Li Y, Ferraiuolo R, Dunning M, Oh S, et al. Neuroendocrine differentiation of prostate cancer leads to PSMA suppression. Endocr Relat Cancer. 2018;26:131-146 pubmed publisher
Yang Hartwich Y, Tedja R, Roberts C, Goodner Bingham J, Cardenas C, Gurea M, et al. p53-Pirh2 Complex Promotes Twist1 Degradation and Inhibits EMT. Mol Cancer Res. 2019;17:153-164 pubmed publisher
Salari A, Thomay K, Lentes J, Ebersold J, Hagedorn M, Skawran B, et al. Effect of TP53 contact and conformational mutations on cell survival and erythropoiesis of human hematopoietic stem cells in a long term culture model. Oncotarget. 2018;9:29869-29876 pubmed publisher
Bernal A, Zafon E, Dominguez D, Bertran E, Tusell L. Generation of Immortalised But Unstable Cells after hTERT Introduction in Telomere-Compromised and p53-Deficient vHMECs. Int J Mol Sci. 2018;19: pubmed publisher
Bernal A, Moltó Abad M, Dominguez D, Tusell L. Acute telomere deprotection prevents ongoing BFB cycles and rampant instability in p16INK4a-deficient epithelial cells. Oncotarget. 2018;9:27151-27170 pubmed publisher
Karlin J, Allen J, Ahmad S, Hughes G, Sheridan V, Odedra R, et al. Orally Bioavailable and Blood-Brain Barrier-Penetrating ATM Inhibitor (AZ32) Radiosensitizes Intracranial Gliomas in Mice. Mol Cancer Ther. 2018;17:1637-1647 pubmed publisher
Pascucci B, D Errico M, Romagnoli A, De Nuccio C, Savino M, Pietraforte D, et al. Overexpression of parkin rescues the defective mitochondrial phenotype and the increased apoptosis of Cockayne Syndrome A cells. Oncotarget. 2017;8:102852-102867 pubmed publisher
Milanovic M, Fan D, Belenki D, Däbritz J, Zhao Z, Yu Y, et al. Senescence-associated reprogramming promotes cancer stemness. Nature. 2018;553:96-100 pubmed publisher
Unno K, Roh M, Yoo Y, Al Shraideh Y, Wang L, Nonn L, et al. Modeling African American prostate adenocarcinoma by inducing defined genetic alterations in organoids. Oncotarget. 2017;8:51264-51276 pubmed publisher
Omar H, Tolba M, Hung J, Al Tel T. OSU-2S/Sorafenib Synergistic Antitumor Combination against Hepatocellular Carcinoma: The Role of PKC?/p53. Front Pharmacol. 2016;7:463 pubmed
Göllner S, Oellerich T, Agrawal Singh S, Schenk T, Klein H, Rohde C, et al. Loss of the histone methyltransferase EZH2 induces resistance to multiple drugs in acute myeloid leukemia. Nat Med. 2017;23:69-78 pubmed publisher
Meitinger F, Anzola J, Kaulich M, Richardson A, Stender J, Benner C, et al. 53BP1 and USP28 mediate p53 activation and G1 arrest after centrosome loss or extended mitotic duration. J Cell Biol. 2016;214:155-66 pubmed publisher
Benatti P, Basile V, Dolfini D, Belluti S, Tomei M, Imbriano C. NF-Y loss triggers p53 stabilization and apoptosis in HPV18-positive cells by affecting E6 transcription. Oncotarget. 2016;7:45901-45915 pubmed publisher
Salari A, Thomay K, Himmler K, Vajen B, Schienke A, Hagedorn M, et al. Establishing a murine xenograft-model for long-term analysis of factors inducing chromosomal instability in myelodysplastic syndrome: Pitfalls and successes. Cancer Genet. 2016;209:258-66 pubmed publisher
Salas E, Roy S, Marsh T, Rubin B, Debnath J. Oxidative pentose phosphate pathway inhibition is a key determinant of antimalarial induced cancer cell death. Oncogene. 2016;35:2913-22 pubmed publisher
Tripathi R, Ash D, Shaha C. Beclin-1-p53 interaction is crucial for cell fate determination in embryonal carcinoma cells. J Cell Mol Med. 2014;18:2275-86 pubmed publisher
Akita H, Marquardt J, Durkin M, Kitade M, Seo D, Conner E, et al. MYC activates stem-like cell potential in hepatocarcinoma by a p53-dependent mechanism. Cancer Res. 2014;74:5903-13 pubmed publisher
Godinho S, Picone R, Burute M, Dagher R, Su Y, Leung C, et al. Oncogene-like induction of cellular invasion from centrosome amplification. Nature. 2014;510:167-71 pubmed publisher
Akdemir K, Jain A, Allton K, Aronow B, Xu X, Cooney A, et al. Genome-wide profiling reveals stimulus-specific functions of p53 during differentiation and DNA damage of human embryonic stem cells. Nucleic Acids Res. 2014;42:205-23 pubmed publisher
Evangelou K, Bartkova J, Kotsinas A, Pateras I, Liontos M, Velimezi G, et al. The DNA damage checkpoint precedes activation of ARF in response to escalating oncogenic stress during tumorigenesis. Cell Death Differ. 2013;20:1485-97 pubmed publisher
Bhatt S, Ashlock B, Toomey N, Diaz L, Mesri E, Lossos I, et al. Efficacious proteasome/HDAC inhibitor combination therapy for primary effusion lymphoma. J Clin Invest. 2013;123:2616-28 pubmed publisher
Jackson T, Salmina K, Huna A, Inashkina I, Jankevics E, Riekstina U, et al. DNA damage causes TP53-dependent coupling of self-renewal and senescence pathways in embryonal carcinoma cells. Cell Cycle. 2013;12:430-41 pubmed publisher
Godar S, Ince T, Bell G, Feldser D, Donaher J, Bergh J, et al. Growth-inhibitory and tumor- suppressive functions of p53 depend on its repression of CD44 expression. Cell. 2008;134:62-73 pubmed publisher
product information
Catalog Number :
19119
Product Name :
shp53 pLKO.1 puro
article :
doi10.1016/j.cell.2008.06.006
id2244
pubmed_id18614011
bacterial resistance :
Ampicillin
cloning :
backbonepLKO.1 puro
backbone_mutation
backbone_originGenHunter
backbone_size7032
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
RNAi
growth notes :
Cloned by Watmick RS.
growth strain :
3rd gen lentiviral vector for knocking down p53 gene expression
growth temp :
Use Stbl3 or HB101 to reduce chance of recombination.
origin :
37
pi :
alt_names
p53, FLJ92943, LFS1, TRP53
cloning
clone_methodRestriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5LKO.1 5'
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBCC7, BMFS5, LFS1, P53, TRP53
geneTP53
id7157
genbank_ids
mutation
namesmall hairpin RNA against tumor protein p53
shRNA_sequence
size
species
9606
Homo sapiens
tags
resistance markers :
568
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA