This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pUC18_BCR_GUSB_e6a2
catalog :
190882
citations: 1
Reference
Sch xe4 fer V, White H, Gerrard G, M xf6 bius S, Saussele S, Franke G, et al. Assessment of individual molecular response in chronic myeloid leukemia patients with atypical BCR-ABL1 fusion transcripts: recommendations by the EUTOS cooperative network. J Cancer Res Clin Oncol. 2021;147:3081-3089 pubmed publisher
product information
Catalog Number :
190882
Product Name :
pUC18_BCR_GUSB_e6a2
article :
doi10.1007/s00432-021-03569-8
id28229368
pubmed_id33677711
bacterial resistance :
Ampicillin
cloning :
backbonepUC18
backbone_mutation
backbone_originUniversity of California
backbone_size2686
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
cloned cDNA transcripts for use in RT-qPCR BCR::ABL1 monitoring of atypical transcripts
growth notes :
Plasmid can be linearised using HindIII
growth strain :
molecular monitoring of atypical BCR::ABL1 fusion transcripts (e6a2)
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5HindIII
promoter
sequencing_primer_3CTT CGT TCT GAG ATA CTG GAT TCC T
sequencing_primer_5CAAAGATGCCAAGGATCCAACGACCAAG
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
namee6a2 cDNA
shRNA_sequence
size1478
species
tags
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3KpnI
cloning_site_5XbaI
promoter
sequencing_primer_3gtaaacgggctgttttccaa
sequencing_primer_5tttccgtaccagccactacc
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBG, MPS7
geneGUSB
id2990
genbank_ids
NM_000181.4
mutation
nameGUSB cDNA transcript
shRNA_sequence
size813
species
tags
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoR1
cloning_site_5EcoRI
promoter
sequencing_primer_3CAAGGACCAGCTGTCAGTCA
sequencing_primer_5GTCCACTCAGCCACTGGATT
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesALL, BCR1, CML, D22S11, D22S662, PHL
geneBCR
id613
genbank_ids
Y00661
mutation
nameBCR cDNA
shRNA_sequence
size963
species
9606
Homo sapiens
tags
resistance markers :
5395
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA