This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pBIG1b_10xHisTEVATG5_ATG12
catalog :
190861
citations: 1
Reference
Fracchiolla D, Chang C, Hurley J, Martens S. A PI3K-WIPI2 positive feedback loop allosterically activates LC3 lipidation in autophagy. J Cell Biol. 2020;219: pubmed publisher
product information
Catalog Number :
190861
Product Name :
pBIG1b_10xHisTEVATG5_ATG12
article :
doi10.1083/jcb.201912098
id28216330
pubmed_id32437499
bacterial resistance :
Ampicillin
cloning :
backbonepBIG1b
backbone_mutation
backbone_originJan-Michael Peters
backbone_size5915
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
Insect Expression
growth notes :
https://hub.asap.science/shared-research/94a2d9aa-2bf9-4325-8a07-513dccf4c4fb
growth strain :
Plasmid for the expression of ATG5 and ATG12 protein subunit complexes. Internal References: SMC1178
origin :
37
pi :
APG5-LIKE, APG5L, ASP, SCAR25, hAPG5geneATG5id9474genbank_ids
NC_000006.12
mutationnameATG5shRNA_sequencesize894species
9606
Homo sapiens
tags
locationN terminal on insert
tag6xHis-TEV
alt_names
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3CCACCATCGGGCGCGGATCCATGGCCGAAGAGCCC
sequencing_primer_5CCACCATCGGGCGCGGATCCATGGCCGAAGAGCCC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAPG12, APG12L, FBR93, HAPG12
geneATG12
id9140
genbank_ids
NC_000005.10
mutation
nameATG12
shRNA_sequence
size423
species
9606
Homo sapiens
tags


alt_names
cloning>Unknown>site_3_destroyed>>
clone_method
cloning_site_
3
cloning_sit
e_5
promoter<
/td>
sequencing_pr
imer_3
TCCTCTAGTACTTCTCGACAAGCT
TTTAGTCCGTAGGTTGGGG
se
quencing_primer_5
CCACCATCGGGCG
CGGATCCATGGCCGAAGAGCCC
site_5_destroyed
entrez_gene<
/td>
a
liases
APG5,
resistance markers :
5424
tags :
Unknown
terms :
Gentamicin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA