This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
ReactionC_pBIG1a_ATG7_ATG10
catalog :
190860
citations: 1
Reference
Fracchiolla D, Chang C, Hurley J, Martens S. A PI3K-WIPI2 positive feedback loop allosterically activates LC3 lipidation in autophagy. J Cell Biol. 2020;219: pubmed publisher
product information
Catalog Number :
190860
Product Name :
ReactionC_pBIG1a_ATG7_ATG10
article :
doi10.1083/jcb.201912098
id28216330
pubmed_id32437499
bacterial resistance :
Ampicillin
cloning :
backbonepBIG1a
backbone_mutation
backbone_originJan-Michael Peters
backbone_size5915
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
Insect Expression
growth notes :
https://hub.asap.science/shared-research/3633829c-35dd-43f5-b9ce-bcb709126a0f
growth strain :
Plasmid for the expression and purification of ATG7 and ATG10 protein subunit complexes. Internal reference: SMC1179
origin :
37
pi :
APG7L, GSA7, SCAR31geneATG7id10533genbank_ids
NC_000003.12
mutationnameATG7shRNA_sequencesize2112species
9606
Homo sapiens
tags
alt_names
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3AACCCCGATTGAGATATAGATTTATTTAAATGGTTATGATAGTTATTGCTCAGCG
sequencing_primer_5CCACCATCGGGCGCGGATCCATGGAGGAGGACGAGTTC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAPG10, APG10L, pp12616
geneATG10
id83734
genbank_ids
NC_000005.10
mutation
nameATG10
shRNA_sequence
size663
species
tags


alt_names
cloning>Unknownd>sequencing_primer_5<
tr>tr>
clone_method
cloning_site_
3
cloning_sit
e_5
promoter<
/td>
sequencing_pr
imer_3
TCCTCTAGTACTTCTCGACAAGCT
TTTAAATAGTCTCATCGTCGCTC
CCACCATCG
GGCGCGGATCCATGGCAGCGGCTACTGG
site_3_destroyed
site_5_destroyedd>
entrez
_gene
>
aliasesAPG7-LIKE,
resistance markers :
5424
tags :
Unknown
terms :
Gentamicin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA