FUGW-APPSwe-T2A-mCherry | Addgene 190804 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

FUGW-APPSwe-T2A-mCherry

catalog :

190804

citations: 1

Reference
Ma F, Akolkar H, Xu J, Liu Y, Popova D, Xie J, et al. The Amyloid Precursor Protein Modulates the Position and Length of the Axon Initial Segment. J Neurosci. 2023;43:1830-1844 pubmed publisher

product information

Catalog Number :

190804

Product Name :

FUGW-APPSwe-T2A-mCherry

article :

doi	10.1523/JNEUROSCI.0172-22.2023
id	28237992
pubmed_id	36717226

bacterial resistance :

Ampicillin

cloning :

backbone

FUGW

backbone_mutation

backbone_origin

backbone_size

9210

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Lentiviral

growth notes :

The human APP coding sequence is from NM_201414, APP variant 3. APP Swedish mutation: KM670/671NL. Reference Assembly: GRCh37 (105)/Position: Chr21:27269939 G>T, Chr21:27269938 A>C. dbSNP ID: rs63751263, rs63750445. Codon Change: AAG.ATG to AAT.CTG

growth strain :

Express Swedish mutant APP from Ubiquitin C promoter, co-expressing mCherry

origin :

pi :

alt_names

cloning

clone_method	Gibson Cloning
cloning_site_3
cloning_site_5
promoter	Ubi C
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, alpha-sAPP, preA4
gene	APP
id	351

genbank_ids

mutation

Swedish (KM670/671NL)

name

APP

shRNA_sequence

size

3108

species

9606

Homo sapiens