This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pPROEX-TwStrp-FTO
catalog :
190265
product information
Catalog Number :
190265
Product Name :
pPROEX-TwStrp-FTO
article :
doi
id28229278
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonepPROEX
backbone_mutation
backbone_originThermoFisher
backbone_size4650
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
Expresses FTO in bacterial cells, with two purification tags: His and Twin-Streptag.
growth temp :
For plasmid induction of FTO - transform in BL21(DE3), add 2mM IPTG and grow overnight at 16 C.
origin :
37
pi :
alt_names
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterTrc
sequencing_primer_3GCGCTACGGCGTTTCACTT
sequencing_primer_5CGGATAACAATTTCACACAG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesALKBH9, BMIQ14, GDFD, IFEX9
geneFTO
id79068
genbank_ids
NM_001080432.3
mutation
nameFTO
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on insert
tag6xHis
locationN terminal on insert
tagTwin-Streptag
resistance markers :
2418
tags :
Unknown
terms :
Other
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA