This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pET-Duet1-6xHis-TEV-LC3BGlydelta5C
catalog :
190237
citations: 1
Reference
Fracchiolla D, Chang C, Hurley J, Martens S. A PI3K-WIPI2 positive feedback loop allosterically activates LC3 lipidation in autophagy. J Cell Biol. 2020;219: pubmed publisher
product information
Catalog Number :
190237
Product Name :
pET-Duet1-6xHis-TEV-LC3BGlydelta5C
article :
doi10.1083/jcb.201912098
id28216330
pubmed_id32437499
bacterial resistance :
Ampicillin
cloning :
backbonepET Duet1
backbone_mutation
backbone_originEMD Biosciences
backbone_size5420
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
https://hub.asap.science/shared-research/1e41d81c-1a19-464c-bdec-522ee6220c8e
growth strain :
Plasmid for the expression and purification of 6xHis-TEV-LC3BGlydelta5C. Internal reference: SMC893.
origin :
37
pi :
alt_names
LC3B
ATG8F
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3GATTATGCGGCCGTGTACAA
sequencing_primer_5ATGCGTCCGGCGTAGA
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesATG8F, LC3B, MAP1A/1BLC3, MAP1LC3B-a
geneMAP1LC3B
id81631
genbank_ids
NC_000016.10
mutation
nameMAP1LC3B
shRNA_sequence
size360
species
9606
Homo sapiens
tags
locationN terminal on insert
tag6xHis-TEV
resistance markers :
5424
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA