This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
AttB_ACE2[R.alcyone+ferrum]-2A-TMPRSS2_IRES-iRFP670-P2A-PuroR
catalog :
190079
citations: 1
Reference
Roelle S, Shukla N, Pham A, Bruchez A, Matreyek K. Expanded ACE2 dependencies of diverse SARS-like coronavirus receptor binding domains. PLoS Biol. 2022;20:e3001738 pubmed publisher
product information
Catalog Number :
190079
Product Name :
AttB_ACE2[R.alcyone+ferrum]-2A-TMPRSS2_IRES-iRFP670-P2A-PuroR
article :
doi10.1371/journal.pbio.3001738
id28229227
pubmed_id35895696
bacterial resistance :
Ampicillin
cloning :
backbonePromoterless Bxb1 recombination vector
backbone_mutation
backbone_origin
backbone_size5971
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Human TMPRSS2 co-expressed with ACE2. Addgene NGS results found a P487R mutation in the P2A region that does not affect plasmid function.
growth strain :
express chimeric Rhinolophus alcyone ACE2 and human TMPRSS2 with selectable markers iRFP670 and puromycin resistance gene
origin :
37
pi :
alt_names
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoternone
sequencing_primer_3CGTTACTGGCCGAAGCCGC
sequencing_primer_5AACGACGGCCAGTGAATTG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesPRSS10
geneTMPRSS2
id7113
genbank_ids
mutation
nameACE2
shRNA_sequence
size3972
species
100
Synthetic
32644
Other
Rhinolophus alcyone and Rhinolophus ferrumequinum
tags
resistance markers :
5508
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA