This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
HA-HIF2alpha-P405A/P531A-pcDNA3
catalog :
18956
citations: 16
Reference
Chao H, Luo J, Hsu M, Chen L, Lu T, Tsai M, et al. Regulatory mechanisms and function of hypoxia-induced long noncoding RNA NDRG1-OT1 in breast cancer cells. Cell Death Dis. 2022;13:807 pubmed publisher
Qin L, Chung Y, Berk M, Naelitz B, Zhu Z, Klein E, et al. Hypoxia-Reoxygenation Couples 3βHSD1 Enzyme and Cofactor Upregulation to Facilitate Androgen Biosynthesis and Hormone Therapy Resistance in Prostate Cancer. Cancer Res. 2022;82:2417-2430 pubmed publisher
Muñiz García A, Romero M, Falcόn Perez J, Murray P, Zorzano A, Mora S. Hypoxia-induced HIF1α activation regulates small extracellular vesicle release in human embryonic kidney cells. Sci Rep. 2022;12:1443 pubmed publisher
Garc xed a Garc xed a C, Acevedo Diaz A, Kumari N, Govindaraju S, DE LA CRUZ BONILLA M, San Lucas F, et al. HIF2 Regulates Intestinal Wnt5a Expression. Front Oncol. 2021;11:769385 pubmed publisher
Shih C, Chuang L, Tsai M, Chen L, Chuang E, Lu T, et al. Hypoxia-Induced MALAT1 Promotes the Proliferation and Migration of Breast Cancer Cells by Sponging MiR-3064-5p. Front Oncol. 2021;11:658151 pubmed publisher
Evagelou S, Bebenek O, Specker E, Uniacke J. DEAD Box Protein Family Member DDX28 Is a Negative Regulator of Hypoxia-Inducible Factor 2α- and Eukaryotic Initiation Factor 4E2-Directed Hypoxic Translation. Mol Cell Biol. 2020;40: pubmed publisher
Rosell García T, Palomo Álvarez O, Rodriguez Pascual F. A hierarchical network of hypoxia-inducible factor and SMAD proteins governs procollagen lysyl hydroxylase 2 induction by hypoxia and transforming growth factor β1. J Biol Chem. 2019;294:14308-14318 pubmed publisher
Kraus R, Yu X, Cordes B, Sathiamoorthi S, Iempridee T, Nawandar D, et al. Hypoxia-inducible factor-1α plays roles in Epstein-Barr virus's natural life cycle and tumorigenesis by inducing lytic infection through direct binding to the immediate-early BZLF1 gene promoter. PLoS Pathog. 2017;13:e1006404 pubmed publisher
Schoepflin Z, Shapiro I, Risbud M. Class I and IIa HDACs Mediate HIF-1? Stability Through PHD2-Dependent Mechanism, While HDAC6, a Class IIb Member, Promotes HIF-1? Transcriptional Activity in Nucleus Pulposus Cells of the Intervertebral Disc. J Bone Miner Res. 2016;31:1287-99 pubmed publisher
Nanayakkara G, Alasmari A, Mouli S, Eldoumani H, Quindry J, McGinnis G, et al. Cardioprotective HIF-1α-frataxin signaling against ischemia-reperfusion injury. Am J Physiol Heart Circ Physiol. 2015;309:H867-79 pubmed publisher
Evensen N, Li Y, Kuscu C, Liu J, Cathcart J, Banach A, et al. Hypoxia promotes colon cancer dissemination through up-regulation of cell migration-inducing protein (CEMIP). Oncotarget. 2015;6:20723-39 pubmed
Becker N, Martitz J, Renko K, Stoedter M, Hybsier S, Cramer T, et al. Hypoxia reduces and redirects selenoprotein biosynthesis. Metallomics. 2014;6:1079-86 pubmed publisher
Wierenga A, Vellenga E, Schuringa J. Convergence of hypoxia and TGF? pathways on cell cycle regulation in human hematopoietic stem/progenitor cells. PLoS ONE. 2014;9:e93494 pubmed publisher
Rider P, Kaplanov I, Romzova M, Bernardis L, Braiman A, Voronov E, et al. The transcription of the alarmin cytokine interleukin-1 alpha is controlled by hypoxia inducible factors 1 and 2 alpha in hypoxic cells. Front Immunol. 2012;3:290 pubmed publisher
Kurihara T, Westenskow P, Krohne T, Aguilar E, Johnson R, Friedlander M. Astrocyte pVHL and HIF-? isoforms are required for embryonic-to-adult vascular transition in the eye. J Cell Biol. 2011;195:689-701 pubmed publisher
Yan Q, Bartz S, Mao M, Li L, Kaelin W. The hypoxia-inducible factor 2alpha N-terminal and C-terminal transactivation domains cooperate to promote renal tumorigenesis in vivo. Mol Cell Biol. 2007;27:2092-102 pubmed
product information
Catalog Number :
18956
Product Name :
HA-HIF2alpha-P405A/P531A-pcDNA3
article :
doi10.1128/MCB.01514-06
id2219
pubmed_id17220275
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3
backbone_mutation
backbone_originInvitrogen
backbone_size5418
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth temp :
DH5alpha
origin :
37
pi :
alt_names
HIF2a
EPAS1
cloning
clone_methodRestriction Enzyme
cloning_site_3Not I
cloning_site_5BamH I
promoter
sequencing_primer_3SP6
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesECYT4, HIF2A, HLF, MOP2, PASD2, bHLHe73
geneEPAS1
id2034
genbank_ids
HSU81984
mutationcDNA changed amino acids: Proline 405 to Alanine Proline 531 to Alanine
nameHypoxia inducible factor 2 alpha
shRNA_sequence
size2631
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagHA-tag
resistance markers :
238
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA