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product summary
company name :
Addgene
product type :
cDNA
product name :
HA-HIF1alpha P402A/P564A-pcDNA3
catalog :
18955
citations: 36
Reference
Rouger Gaudichon J, Cousin E, Jakobczyk H, Debaize L, Rio A, Forestier A, et al. Hypoxia regulates CD9 expression and dissemination of B lymphoblasts. Leuk Res. 2022;123:106964 pubmed publisher
Chao H, Luo J, Hsu M, Chen L, Lu T, Tsai M, et al. Regulatory mechanisms and function of hypoxia-induced long noncoding RNA NDRG1-OT1 in breast cancer cells. Cell Death Dis. 2022;13:807 pubmed publisher
Wu Y, Tang X, Lee S, Hong H, Cao X, Lau C, et al. Endothelial PPARδ facilitates the post-ischemic vascular repair through interaction with HIF1α. Theranostics. 2022;12:1855-1869 pubmed publisher
Medeiros P, Pascetta S, Kirsh S, Al Khazraji B, Uniacke J. Expression of hypoxia inducible factor-dependent neuropeptide Y receptors Y1 and Y5 sensitizes hypoxic cells to NPY stimulation. J Biol Chem. 2022;298:101645 pubmed publisher
Muñiz García A, Romero M, Falcόn Perez J, Murray P, Zorzano A, Mora S. Hypoxia-induced HIF1α activation regulates small extracellular vesicle release in human embryonic kidney cells. Sci Rep. 2022;12:1443 pubmed publisher
Garc xed a Garc xed a C, Acevedo Diaz A, Kumari N, Govindaraju S, DE LA CRUZ BONILLA M, San Lucas F, et al. HIF2 Regulates Intestinal Wnt5a Expression. Front Oncol. 2021;11:769385 pubmed publisher
Saha P, Kim M, Tulshyan A, Guo Y, Mishra R, Li D, et al. Hypoxia-inducible factor 1-alpha enhances the secretome to rejuvenate adult cardiosphere-derived cells. J Thorac Cardiovasc Surg. 2021;: pubmed publisher
Shih C, Chuang L, Tsai M, Chen L, Chuang E, Lu T, et al. Hypoxia-Induced MALAT1 Promotes the Proliferation and Migration of Breast Cancer Cells by Sponging MiR-3064-5p. Front Oncol. 2021;11:658151 pubmed publisher
Riedl T, Faure Dupuy S, Rolland M, Schuehle S, Hizir Z, Calderazzo S, et al. HIF1α-mediated RelB/APOBEC3B downregulation allows Hepatitis B Virus persistence. Hepatology. 2021;: pubmed publisher
Li Z, Wang B, Lv L, Tang T, Wen Y, Cao J, et al. FIH-1-modulated HIF-1α C-TAD promotes acute kidney injury to chronic kidney disease progression via regulating KLF5 signaling. Acta Pharmacol Sin. 2021;: pubmed publisher
Jin X, Gong L, Peng Y, Li L, Liu G. Enhancer-bound Nrf2 licenses HIF-1α transcription under hypoxia to promote cisplatin resistance in hepatocellular carcinoma cells. Aging (Albany NY). 2020;13:364-375 pubmed publisher
Est Witte S, Farris A, Tzeng S, Hutton D, Gong D, Calabresi K, et al. Non-viral gene delivery of HIF-1α promotes angiogenesis in human adipose-derived stem cells. Acta Biomater. 2020;: pubmed publisher
Evagelou S, Bebenek O, Specker E, Uniacke J. DEAD Box Protein Family Member DDX28 Is a Negative Regulator of Hypoxia-Inducible Factor 2α- and Eukaryotic Initiation Factor 4E2-Directed Hypoxic Translation. Mol Cell Biol. 2020;40: pubmed publisher
Khan H, Anshu A, Prasad A, Roy S, Jeffery J, Kittipongdaja W, et al. Metabolic Rewiring in Response to Biguanides Is Mediated by mROS/HIF-1a in Malignant Lymphocytes. Cell Rep. 2019;29:3009-3018.e4 pubmed publisher
Rosell García T, Palomo Álvarez O, Rodriguez Pascual F. A hierarchical network of hypoxia-inducible factor and SMAD proteins governs procollagen lysyl hydroxylase 2 induction by hypoxia and transforming growth factor β1. J Biol Chem. 2019;294:14308-14318 pubmed publisher
Harrison H, Pegg H, Thompson J, Bates C, Shore P. HIF1-alpha expressing cells induce a hypoxic-like response in neighbouring cancer cells. BMC Cancer. 2018;18:674 pubmed publisher
Rosner M, Pham H, Moriggl R, Hengstschlager M. Human stem cells alter the invasive properties of somatic cells via paracrine activation of mTORC1. Nat Commun. 2017;8:595 pubmed publisher
Park C, Ivanova I, Kenneth N. XIAP upregulates expression of HIF target genes by targeting HIF1α for Lys63-linked polyubiquitination. Nucleic Acids Res. 2017;45:9336-9347 pubmed publisher
Kraus R, Yu X, Cordes B, Sathiamoorthi S, Iempridee T, Nawandar D, et al. Hypoxia-inducible factor-1α plays roles in Epstein-Barr virus's natural life cycle and tumorigenesis by inducing lytic infection through direct binding to the immediate-early BZLF1 gene promoter. PLoS Pathog. 2017;13:e1006404 pubmed publisher
Im S, Kim D. Nkx3.2 induces oxygen concentration-independent and lysosome-dependent degradation of HIF-1? to modulate hypoxic responses in chondrocytes. Cell Signal. 2017;36:127-138 pubmed publisher
Mittal K, Choi D, Ogden A, Donthamsetty S, Melton B, Gupta M, et al. Amplified centrosomes and mitotic index display poor concordance between patient tumors and cultured cancer cells. Sci Rep. 2017;7:43984 pubmed publisher
Lin M, Lin J, Hsu C, Juan H, Lou P, Huang M. GATA3 interacts with and stabilizes HIF-1α to enhance cancer cell invasiveness. Oncogene. 2017;36:4243-4252 pubmed publisher
Lee K, Yim H, Shin J, Lee C, Lee J, Jeong J. FGF11 induced by hypoxia interacts with HIF-1? and enhances its stability. FEBS Lett. 2017;591:348-357 pubmed publisher
Baumann B, Hayashida T, Liang X, Schnaper H. Hypoxia-inducible factor-1α promotes glomerulosclerosis and regulates COL1A2 expression through interactions with Smad3. Kidney Int. 2016;90:797-808 pubmed publisher
Sun Y, Guan Z, Liang L, Cheng Y, Zhou J, Li J, et al. HIF-1α/MDR1 pathway confers chemoresistance to cisplatin in bladder cancer. Oncol Rep. 2016;35:1549-56 pubmed publisher
Dey P, Velázquez Villegas L, Faria M, Turner A, Jonsson P, Webb P, et al. Estrogen Receptor β2 Induces Hypoxia Signature of Gene Expression by Stabilizing HIF-1α in Prostate Cancer. PLoS ONE. 2015;10:e0128239 pubmed publisher
Joshi H, Subramanian I, Schnettler E, Ghosh G, Rupaimoole R, Evans C, et al. Dynamin 2 along with microRNA-199a reciprocally regulate hypoxia-inducible factors and ovarian cancer metastasis. Proc Natl Acad Sci U S A. 2014;111:5331-6 pubmed publisher
Becker N, Martitz J, Renko K, Stoedter M, Hybsier S, Cramer T, et al. Hypoxia reduces and redirects selenoprotein biosynthesis. Metallomics. 2014;6:1079-86 pubmed publisher
Wierenga A, Vellenga E, Schuringa J. Convergence of hypoxia and TGF? pathways on cell cycle regulation in human hematopoietic stem/progenitor cells. PLoS ONE. 2014;9:e93494 pubmed publisher
Gupta Saraf P, Miller C. HIF-1? downregulation and apoptosis in hypoxic prostate tumor cells infected with oncolytic mammalian orthoreovirus. Oncotarget. 2014;5:561-74 pubmed
Bhalla S, Evens A, Prachand S, Schumacker P, Gordon L. Paradoxical regulation of hypoxia inducible factor-1? (HIF-1?) by histone deacetylase inhibitor in diffuse large B-cell lymphoma. PLoS ONE. 2013;8:e81333 pubmed publisher
Tran C, Fujita N, Huang B, Ong J, Lyons K, Shapiro I, et al. Hypoxia-inducible factor (HIF)-1? and CCN2 form a regulatory circuit in hypoxic nucleus pulposus cells: CCN2 suppresses HIF-1? level and transcriptional activity. J Biol Chem. 2013;288:12654-66 pubmed publisher
Rider P, Kaplanov I, Romzova M, Bernardis L, Braiman A, Voronov E, et al. The transcription of the alarmin cytokine interleukin-1 alpha is controlled by hypoxia inducible factors 1 and 2 alpha in hypoxic cells. Front Immunol. 2012;3:290 pubmed publisher
Fujita N, Markova D, Anderson D, Chiba K, Toyama Y, Shapiro I, et al. Expression of prolyl hydroxylases (PHDs) is selectively controlled by HIF-1 and HIF-2 proteins in nucleus pulposus cells of the intervertebral disc: distinct roles of PHD2 and PHD3 proteins in controlling HIF-1? activity in hypoxia. J Biol Chem. 2012;287:16975-86 pubmed publisher
Kurihara T, Westenskow P, Krohne T, Aguilar E, Johnson R, Friedlander M. Astrocyte pVHL and HIF-? isoforms are required for embryonic-to-adult vascular transition in the eye. J Cell Biol. 2011;195:689-701 pubmed publisher
Yan Q, Bartz S, Mao M, Li L, Kaelin W. The hypoxia-inducible factor 2alpha N-terminal and C-terminal transactivation domains cooperate to promote renal tumorigenesis in vivo. Mol Cell Biol. 2007;27:2092-102 pubmed
product information
Catalog Number :
18955
Product Name :
HA-HIF1alpha P402A/P564A-pcDNA3
article :
doi10.1128/MCB.01514-06
id2219
pubmed_id17220275
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3
backbone_mutation
backbone_originInvitrogen
backbone_size5433
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth temp :
DH5alpha
origin :
37
pi :
alt_names
HIF1a
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoR I
cloning_site_5BamH I
promoter
sequencing_primer_3SP6
sequencing_primer_5T7
site_3_destroyed1
site_5_destroyed
entrez_gene
aliasesHIF-1-alpha, HIF-1A, HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78
geneHIF1A
id3091
genbank_ids
NM_001530
mutationcDNA changed amino acids: Proline 402 to Alanine Proline 564 to Alanine
nameHypoxia inducible factor 1 alpha
shRNA_sequence
size2505
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagHA-tag
resistance markers :
238
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA