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product summary
company name :
Addgene
product type :
cDNA
product name :
HA-HIF1alpha-pcDNA3
catalog :
18949
citations: 52
Reference
Ali M, Khramushin A, Yadav V, Schueler Furman O, Ivarsson Y. Elucidation of Short Linear Motif-Based Interactions of the FERM Domains of Ezrin, Radixin, Moesin, and Merlin. Biochemistry. 2023;62:1594-1607 pubmed publisher
Zou J, Wang W, Lu Y, Ayala J, Dong K, Zhou H, et al. Neddylation is required for perinatal cardiac development through stimulation of metabolic maturation. Cell Rep. 2023;42:112018 pubmed publisher
Simmler P, Cortijo C, Koch L, Galliker P, Angori S, Bolck H, et al. SF3B1 facilitates HIF1-signaling and promotes malignancy in pancreatic cancer. Cell Rep. 2022;40:111266 pubmed publisher
Jacquier V, Gitenay D, Fritsch S, Bonnet S, Gyorffy B, Jalaguier S, et al. RIP140 inhibits glycolysis-dependent proliferation of breast cancer cells by regulating GLUT3 expression through transcriptional crosstalk between hypoxia induced factor and p53. Cell Mol Life Sci. 2022;79:270 pubmed publisher
Nelson J, Thin M, Evan T, Howell S, Wu M, Almeida B, et al. USP25 promotes pathological HIF-1-driven metabolic reprogramming and is a potential therapeutic target in pancreatic cancer. Nat Commun. 2022;13:2070 pubmed publisher
Hauffe L, Picard D, Musa J, Remke M, Grunewald T, Rotblat B, et al. Eukaryotic translation initiation factor 4E binding protein 1 (EIF4EBP1) expression in glioblastoma is driven by ETS1- and MYBL2-dependent transcriptional activation. Cell Death Discov. 2022;8:91 pubmed publisher
Wu Y, Tang X, Lee S, Hong H, Cao X, Lau C, et al. Endothelial PPARδ facilitates the post-ischemic vascular repair through interaction with HIF1α. Theranostics. 2022;12:1855-1869 pubmed publisher
Döhla J, Kuuluvainen E, Gebert N, Amaral A, Englund J, Gopalakrishnan S, et al. Metabolic determination of cell fate through selective inheritance of mitochondria. Nat Cell Biol. 2022;24:148-154 pubmed publisher
Mallet C, Cochard J, Leclercq S, Trapp Fragnet L, Chouteau P, Denesvre C. Hypoxia and HIF-1 Trigger Marek's Disease Virus Reactivation in Lymphoma-Derived Latently Infected T Lymphocytes. J Virol. 2021;:JVI0142721 pubmed publisher
Kim J, An H, Yeo H, Jeong Y, Lee H, Lee J, et al. Activation of Hypoxia-Inducible Factor-1α Signaling Pathway Has the Protective Effect of Intervertebral Disc Degeneration. Int J Mol Sci. 2021;22: pubmed publisher
Zhao S, Zhou L, Dicker D, Lev A, Zhang S, Ross E, et al. Anti-cancer efficacy including Rb-deficient tumors and VHL-independent HIF1α proteasomal destabilization by dual targeting of CDK1 or CDK4/6 and HSP90. Sci Rep. 2021;11:20871 pubmed publisher
Kumar P, Verma V, Mohania D, Gupta S, Babbar A, Rathi B, et al. Leukemia associated RUNX1T1 gene reduced proliferation and invasiveness of glioblastoma cells. J Cell Biochem. 2021;: pubmed publisher
Casillas A, Chauhan S, Toth R, Sainz A, Clements A, Jensen C, et al. Direct phosphorylation and stabilization of HIF-1α by PIM1 kinase drives angiogenesis in solid tumors. Oncogene. 2021;40:5142-5152 pubmed publisher
Ivanova E, Sharma S, Brichkina A, Pfefferle P, Keber U, Pagenstecher A, et al. DYRK3 contributes to differentiation and hypoxic control in neuroblastoma. Biochem Biophys Res Commun. 2021;567:215-221 pubmed publisher
Silwal P, Kim J, Jeon S, Lee J, Kim Y, Kim Y, et al. Mitofusin-2 boosts innate immunity through the maintenance of aerobic glycolysis and activation of xenophagy in mice. Commun Biol. 2021;4:548 pubmed publisher
Wise L, Xi Y, Purdy J. Hypoxia-Inducible Factor 1α (HIF1α) Suppresses Virus Replication in Human Cytomegalovirus Infection by Limiting Kynurenine Synthesis. MBio. 2021;12: pubmed publisher
Lu J, Chen H, He F, You Y, Feng Z, Chen W, et al. Ginsenoside 20(S)-Rg3 upregulates HIF-1α-targeting miR-519a-5p to inhibit the Warburg effect in ovarian cancer cells. Clin Exp Pharmacol Physiol. 2020;47:1455-1463 pubmed publisher
Yoo H, Park S, Nam M, Kang J, Kim K, Yeo J, et al. A Variant of SLC1A5 Is a Mitochondrial Glutamine Transporter for Metabolic Reprogramming in Cancer Cells. Cell Metab. 2019;: pubmed publisher
Khan H, Anshu A, Prasad A, Roy S, Jeffery J, Kittipongdaja W, et al. Metabolic Rewiring in Response to Biguanides Is Mediated by mROS/HIF-1a in Malignant Lymphocytes. Cell Rep. 2019;29:3009-3018.e4 pubmed publisher
Soyal S, Bonova P, Kwik M, Zara G, Auer S, Scharler C, et al. The Expression of CNS-Specific PPARGC1A Transcripts Is Regulated by Hypoxia and a Variable GT Repeat Polymorphism. Mol Neurobiol. 2019;: pubmed publisher
Faria M, Shepherd P, Pan Y, Chatterjee S, Navone N, Gustafsson J, et al. The estrogen receptor variants β2 and β5 induce stem cell characteristics and chemotherapy resistance in prostate cancer through activation of hypoxic signaling. Oncotarget. 2018;9:36273-36288 pubmed publisher
Seira N, Yamagata K, Fukushima K, Araki Y, Kurata N, Yanagisawa N, et al. Cellular density-dependent increases in HIF-1α compete with c-Myc to down-regulate human EP4 receptor promoter activity through Sp-1-binding region. Pharmacol Res Perspect. 2018;6:e00441 pubmed publisher
Bae Y, Joo H, Bae J, Hyeon S, Her S, Ko E, et al. Brain injury induces HIF-1α-dependent transcriptional activation of LRRK2 that exacerbates brain damage. Cell Death Dis. 2018;9:1125 pubmed publisher
Field J, Martens M, Mughal W, Hai Y, Chapman D, Hatch G, et al. Misoprostol regulates Bnip3 repression and alternative splicing to control cellular calcium homeostasis during hypoxic stress. Cell Death Discov. 2018;4:37 pubmed publisher
Senavirathna L, Huang C, Yang X, Munteanu M, Sathiaseelan R, Xu D, et al. Hypoxia induces pulmonary fibroblast proliferation through NFAT signaling. Sci Rep. 2018;8:2709 pubmed publisher
Sperling S, Aung T, Martin S, Rohde V, Ninkovic M. Riluzole: a potential therapeutic intervention in human brain tumor stem-like cells. Oncotarget. 2017;8:96697-96709 pubmed publisher
Jeon D, Park H, Kim H. Protein S-glutathionylation induced by hypoxia increases hypoxia-inducible factor-1? in human colon cancer cells. Biochem Biophys Res Commun. 2018;495:212-216 pubmed publisher
Xu L, Li H, Wu L, Huang S. YBX1 promotes tumor growth by elevating glycolysis in human bladder cancer. Oncotarget. 2017;8:65946-65956 pubmed publisher
Rosner M, Pham H, Moriggl R, Hengstschlager M. Human stem cells alter the invasive properties of somatic cells via paracrine activation of mTORC1. Nat Commun. 2017;8:595 pubmed publisher
Park C, Ivanova I, Kenneth N. XIAP upregulates expression of HIF target genes by targeting HIF1α for Lys63-linked polyubiquitination. Nucleic Acids Res. 2017;45:9336-9347 pubmed publisher
Lee K, Yim H, Shin J, Lee C, Lee J, Jeong J. FGF11 induced by hypoxia interacts with HIF-1? and enhances its stability. FEBS Lett. 2017;591:348-357 pubmed publisher
Diaz Trelles R, Scimia M, Bushway P, Tran D, Monosov A, Monosov E, et al. Notch-independent RBPJ controls angiogenesis in the adult heart. Nat Commun. 2016;7:12088 pubmed publisher
Wang F, Zhang H, Xu N, Huang N, Tian C, Ye A, et al. A novel hypoxia-induced miR-147a regulates cell proliferation through a positive feedback loop of stabilizing HIF-1α. Cancer Biol Ther. 2016;17:790-8 pubmed publisher
Luo B, Wang M, Hou N, Hu X, Jia G, Qin X, et al. ATP-Dependent Lon Protease Contributes to Helicobacter pylori-Induced Gastric Carcinogenesis. Neoplasia. 2016;18:242-52 pubmed publisher
Suico M, Taura M, Kudo E, Gotoh K, Shuto T, Okada S, et al. The ETS Factor Myeloid Elf-1-Like Factor (MEF)/Elf4 Is Transcriptionally and Functionally Activated by Hypoxia. Biol Pharm Bull. 2016;39:641-7 pubmed publisher
Takarada T, Kou M, Hida M, Fukumori R, Nakamura S, Kutsukake T, et al. Protective upregulation of activating transcription factor-3 against glutamate neurotoxicity in neuronal cells under ischemia. J Neurosci Res. 2016;94:378-88 pubmed publisher
Azoitei N, Becher A, Steinestel K, Rouhi A, Diepold K, Genze F, et al. PKM2 promotes tumor angiogenesis by regulating HIF-1α through NF-κB activation. Mol Cancer. 2016;15:3 pubmed publisher
Erapaneedi R, Belousov V, Schäfers M, Kiefer F. A novel family of fluorescent hypoxia sensors reveal strong heterogeneity in tumor hypoxia at the cellular level. EMBO J. 2016;35:102-13 pubmed publisher
No Y, Lee S, Kumar A, Yun C. HIF1α-Induced by Lysophosphatidic Acid Is Stabilized via Interaction with MIF and CSN5. PLoS ONE. 2015;10:e0137513 pubmed publisher
Dey P, Velázquez Villegas L, Faria M, Turner A, Jonsson P, Webb P, et al. Estrogen Receptor β2 Induces Hypoxia Signature of Gene Expression by Stabilizing HIF-1α in Prostate Cancer. PLoS ONE. 2015;10:e0128239 pubmed publisher
Takarada T, Ogura M, Nakamichi N, Kakuda T, Nakazato R, Kokubo H, et al. Upregulation of Slc38a1 Gene Along with Promotion of Neurosphere Growth and Subsequent Neuronal Specification in Undifferentiated Neural Progenitor Cells Exposed to Theanine. Neurochem Res. 2016;41:5-15 pubmed publisher
Liu Y, Chen C, Qian P, Lu X, Sun B, Zhang X, et al. Gd-metallofullerenol nanomaterial as non-toxic breast cancer stem cell-specific inhibitor. Nat Commun. 2015;6:5988 pubmed publisher
Hou P, Kuo C, Cheng C, Liou J, Ann D, Chen Q. Intermediary metabolite precursor dimethyl-2-ketoglutarate stabilizes hypoxia-inducible factor-1α by inhibiting prolyl-4-hydroxylase PHD2. PLoS ONE. 2014;9:e113865 pubmed publisher
Clarke R, Moosa S, Anzivino M, Wang Y, Floyd D, Purow B, et al. Sustained radiosensitization of hypoxic glioma cells after oxygen pretreatment in an animal model of glioblastoma and in vitro models of tumor hypoxia. PLoS ONE. 2014;9:e111199 pubmed publisher
Qi L, Zhu F, Li S, Si L, Hu L, Tian H. Retinoblastoma binding protein 2 (RBP2) promotes HIF-1α-VEGF-induced angiogenesis of non-small cell lung cancer via the Akt pathway. PLoS ONE. 2014;9:e106032 pubmed publisher
Imagawa K, de Andrés M, Hashimoto K, Itoi E, Otero M, Roach H, et al. Association of reduced type IX collagen gene expression in human osteoarthritic chondrocytes with epigenetic silencing by DNA hypermethylation. Arthritis Rheumatol. 2014;66:3040-51 pubmed publisher
Seo K, Park J, Heo J, Jing K, Han J, Min K, et al. SIRT2 regulates tumour hypoxia response by promoting HIF-1? hydroxylation. Oncogene. 2015;34:1354-62 pubmed publisher
Gupta Saraf P, Miller C. HIF-1? downregulation and apoptosis in hypoxic prostate tumor cells infected with oncolytic mammalian orthoreovirus. Oncotarget. 2014;5:561-74 pubmed
Zhu J, Jiang X, Chehab F. FoxO4 interacts with the sterol regulatory factor SREBP2 and the hypoxia inducible factor HIF2? at the CYP51 promoter. J Lipid Res. 2014;55:431-42 pubmed publisher
Guan Z, Ding C, Du Y, Zhang K, Zhu J, Zhang T, et al. HAF drives the switch of HIF-1α to HIF-2α by activating the NF-κB pathway, leading to malignant behavior of T24 bladder cancer cells. Int J Oncol. 2014;44:393-402 pubmed publisher
Samarajeewa N, Yang F, Docanto M, Sakurai M, McNamara K, Sasano H, et al. HIF-1? stimulates aromatase expression driven by prostaglandin E2 in breast adipose stroma. Breast Cancer Res. 2013;15:R30 pubmed publisher
Kondo K, Klco J, Nakamura E, Lechpammer M, Kaelin W. Inhibition of HIF is necessary for tumor suppression by the von Hippel-Lindau protein. Cancer Cell. 2002;1:237-46 pubmed
product information
Catalog Number :
18949
Product Name :
HA-HIF1alpha-pcDNA3
article :
doi10.1016/S1535-6108(02)00043-0
id2216
pubmed_id12086860
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3
backbone_mutation
backbone_originInvitrogen
backbone_size5433
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth temp :
DH5alpha
origin :
37
pi :
alt_names
HIF1a
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoR I
cloning_site_5BamH I
promoter
sequencing_primer_3SP6
sequencing_primer_5T7
site_3_destroyed1
site_5_destroyed
entrez_gene
aliasesHIF-1-alpha, HIF-1A, HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78
geneHIF1A
id3091
genbank_ids
NM_001530
mutationwt cDNA
nameHypoxia inducible factor 1 alpha
shRNA_sequence
size2505
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagHA-tag
resistance markers :
238
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA