This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pET16b-Mms2
catalog :
18893
citations: 3
Reference
Martinez Fonts K, Matouschek A. A Rapid and Versatile Method for Generating Proteins with Defined Ubiquitin Chains. Biochemistry. 2016;55:1898-908 pubmed publisher
Cannon J, Martinez Fonts K, Robotham S, Matouschek A, Brodbelt J. Top-down 193-nm ultraviolet photodissociation mass spectrometry for simultaneous determination of polyubiquitin chain length and topology. Anal Chem. 2015;87:1812-20 pubmed publisher
Raasi S, Pickart C. Ubiquitin chain synthesis. Methods Mol Biol. 2005;301:47-55 pubmed
product information
Catalog Number :
18893
Product Name :
pET16b-Mms2
article :
doi10.1385/1-59259-895-1:047
id2199
pubmed_id15917625
bacterial resistance :
Ampicillin
cloning :
backbonepET16b
backbone_mutation
backbone_origin
backbone_size5700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Hofmann R.M. Pickart C.M. 1999, Cell 96, 645-653
growth temp :
BL21pJY2
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5NdeI
promoter
sequencing_primer_3T7
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYGL087C
geneMMS2
id852793
genbank_ids
mutation
nameYeast ubiquitin conjugating enzyme Mms2
shRNA_sequence
size
species
4932
Saccharomyces cerevisiae
tags
locationN terminal on backbone
tagHIS10
plasmid copy :
This plasmid is constructed by Hofmann R.M.
resistance markers :
392
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA