This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
PYBS375
catalog :
18887
citations: 1
Reference
Choi K, Satterberg B, Lyons D, Elion E. Ste5 tethers multiple protein kinases in the MAP kinase cascade required for mating in S. cerevisiae. Cell. 1994;78:499-512 pubmed
product information
Catalog Number :
18887
Product Name :
PYBS375
article :
doi10.1016/0092-8674(94)90427-8
id2196
pubmed_id8062390
bacterial resistance :
Ampicillin
cloning :
backbonepYBS317
backbone_mutation
backbone_origin
backbone_size11000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
growth notes :
Lex-Ste11 has the 0.9kb PflMI-SalI fragment of pYBS345 (the PCR-derived Ste11 in pYBS317; see article) deleted by blunt-end ligation with Klenow. The open reading frame reads through to an unknown stop in the vector.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5PflMI
promoter
sequencing_primer_3pHybLex-rev
sequencing_primer_5pHybLex_fwd
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesYLR362W
geneSTE11
id851076
genbank_ids
mutationLex-Ste11C (amino-terminal 413 amino acids)
nameSte11
shRNA_sequence
size1239
species
4932
Saccharomyces cerevisiae
tags
resistance markers :
132
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA