pEX-SP-YFP-STIM1(D76A) | Addgene 18859 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pEX-SP-YFP-STIM1(D76A)

catalog :

18859

citations: 4

Reference
Tolstykh G, Cantu J, Tarango M, Ibey B. Receptor- and store-operated mechanisms of calcium entry during the nanosecond electric pulse-induced cellular response. Biochim Biophys Acta Biomembr. 2019;1861:685-696 pubmed publisher
Parry D, Holmes T, Gamper N, El Sayed W, Hettiarachchi N, Ahmed M, et al. A homozygous STIM1 mutation impairs store-operated calcium entry and natural killer cell effector function without clinical immunodeficiency. J Allergy Clin Immunol. 2016;137:955-7.e8 pubmed publisher
Dingsdale H, Okeke E, Awais M, Haynes L, Criddle D, Sutton R, et al. Saltatory formation, sliding and dissolution of ER-PM junctions in migrating cancer cells. Biochem J. 2013;451:25-32 pubmed publisher
Liou J, Kim M, Heo W, Jones J, Myers J, Ferrell J, et al. STIM is a Ca2+ sensor essential for Ca2+-store-depletion-triggered Ca2+ influx. Curr Biol. 2005;15:1235-41 pubmed

product information

Catalog Number :

18859

Product Name :

pEX-SP-YFP-STIM1(D76A)

article :

doi	10.1016/j.cub.2005.05.055
id	2187
pubmed_id	16005298

bacterial resistance :

Kanamycin

cloning :

backbone

Gateway destination

backbone_mutation

backbone_origin

Invitrogen

backbone_size

5000

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

origin :

pi :

alt_names

STIM1

cloning

clone_method	Gateway Cloning
cloning_site_3	attB2
cloning_site_5	attB1
promoter
sequencing_primer_3	n/a
sequencing_primer_5	n/a
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	D11S4896E, GOK, IMD10, STRMK, TAM, TAM1
gene	STIM1
id	6786

genbank_ids

NM_003156

mutation

D76A mutation - changed aspartate 76 to alanine. YFP is inserted between residues 1-23 (signal peptide) and residues 23-685 of STIM1. Lab plasmid MK2007.

name

Stromal interaction molecule 1

shRNA_sequence

size

1995

species

9606

Homo sapiens