This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Cdk2-DN-HA
catalog :
1885
citations: 5
Reference
Zhang H, Pandey S, Travers M, Sun H, Morton G, Madzo J, et al. Targeting CDK9 Reactivates Epigenetically Silenced Genes in Cancer. Cell. 2018;175:1244-1258.e26 pubmed publisher
Polanco M, Parodi S, Piol D, Stack C, Chivet M, Contestabile A, et al. Adenylyl cyclase activating polypeptide reduces phosphorylation and toxicity of the polyglutamine-expanded androgen receptor in spinobulbar muscular atrophy. Sci Transl Med. 2016;8:370ra181 pubmed publisher
Pfister J, D Mello S. Regulation of Neuronal Survival by Nucleophosmin 1 (NPM1) Is Dependent on Its Expression Level, Subcellular Localization, and Oligomerization Status. J Biol Chem. 2016;291:20787-97 pubmed publisher
Rizzolio F, Lucchetti C, Caligiuri I, Marchesi I, Caputo M, Klein Szanto A, et al. Retinoblastoma tumor-suppressor protein phosphorylation and inactivation depend on direct interaction with Pin1. Cell Death Differ. 2012;19:1152-61 pubmed publisher
van den Heuvel S, Harlow E. Distinct roles for cyclin-dependent kinases in cell cycle control. Science. 1993;262:2050-4 pubmed
product information
Catalog Number :
1885
Product Name :
Cdk2-DN-HA
article :
doi10.1126/science.8266103
id114
pubmed_id8266103
bacterial resistance :
Ampicillin
cloning :
backbonepCMV-neo-Bam
backbone_mutation
backbone_origin
backbone_size6550
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
See scanned map. Addgene's NGS results confirmed that the noted mutation is D145N compared to NP_001789.2.
origin :
37
pi :
alt_names
cyclin-dependent kinase 2
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5BamHI
promoter
sequencing_primer_3Bglob-pA-R
sequencing_primer_5Bglob-intron-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCDKN2, p33(CDK2)
geneCDK2
id1017
genbank_ids
mutationD146N, dominant negative
nameCdk2
shRNA_sequence
size900
species
9606
Homo sapiens
tags
locationC terminal on insert
tagHA
resistance markers :
544
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA