This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
K365-micro
catalog :
188457
citations: 1
Reference
Lemma L, Varghese M, Ross T, Thomson M, Baskaran A, Dogic Z. Spatio-temporal patterning of extensile active stresses in microtubule-based active fluids. PNAS Nexus. 2023;2:pgad130 pubmed publisher
product information
Catalog Number :
188457
Product Name :
K365-micro
article :
doi10.1093/pnasnexus/pgad130
id28238413
pubmed_id37168671
bacterial resistance :
Ampicillin
cloning :
backbonepQE-80L
backbone_mutation
backbone_originQiagen
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Plasmid was derived from plasmids made by Jeff Gelles, Brian Kulman and Matt Thomson. This plasmid was created with Tyler Ross and Matt Thomson.
growth strain :
Component of optically-induced motor dimerization system
origin :
37
pi :
2R6, CG7765, DK, DKH, Dm KHC, DmK, DmKHC, Dmel CG7765, Dmkin, KHC, KIF 5A, KIF5, KIF5B, KIN, Kif5, Kin, Kin-1, Kinesin-1, khc, kin, kinesin, kinesin-1, l(2)W12, l(2)k13219, l(2)k13314, l(2R)W12, pgsgeneKhcid36810genbank_idsmutationKinesin amino acids 1-365nameKinesin 1-365shRNA_sequencesizespecies
7227
Drosophila melanogaster
tags
locationN terminal on insert
tag6xHis-MBP-TEV
locationC terminal on insert
tagSspB micro

Cloningcloning_site_3
cloning_site
_5promotertd>T7sequencing_p
rimer_3CTTACCCTTTAGTCGGGCGTTC<
/td>sequencing_primer_5d>GAACGCCCGACTAAAGGGTAAGggtagtggtag
tggatccagctccccgaaacgc>site_3_destroyed>site_5_destroyed>entrez_gene<
/td>
a
liases		Dmel_CG7765,
alt_names
Kinesin heavy chain
His MBP K365 SspB micro
cloning
clone_methodGibson
resistance markers :
6107
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA