This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
His-FLAG-Tev_ACTB (beta-actin)
catalog :
188453
citations: 1
Reference
Ceron R, Carman P, Rebowski G, Boczkowska M, Heuckeroth R, Dominguez R. A solution to the long-standing problem of actin expression and purification. Proc Natl Acad Sci U S A. 2022;119:e2209150119 pubmed publisher
product information
Catalog Number :
188453
Product Name :
His-FLAG-Tev_ACTB (beta-actin)
article :
doi10.1073/pnas.2209150119
id28229033
pubmed_id36197995
bacterial resistance :
Kanamycin
cloning :
backbonepJCX4
backbone_mutation
backbone_origin
backbone_size4672
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Express in mammalian cells human beta-actin (ACTB) with N-terminal 6xHis tag, FLAG tag, and TEV protease site.
origin :
37
pi :
alt_names
beta cytoplasmic actin
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5SpeI
promoterCMV
sequencing_primer_3WPRE_rev
sequencing_primer_5CMV_for
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBKRNS, BNS, BRWS1, CSMH, DDS1, PS1TP5BP1, THC8
geneACTB
id60
genbank_ids
mutationWT
nameACTB
shRNA_sequence
size1122
species
9606
Homo sapiens
tags
locationN terminal on insert
tag6xHis-FLAG
resistance markers :
5490
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA