This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pDR111_mannose_Stat1Klf6
catalog :
188400
citations: 1
Reference
Madsen C, Makela A, Greeson E, Hardy J, Contag C. Engineered endosymbionts that alter mammalian cell surface marker, cytokine and chemokine expression. Commun Biol. 2022;5:888 pubmed publisher
product information
Catalog Number :
188400
Product Name :
pDR111_mannose_Stat1Klf6
article :
doi10.1038/s42003-022-03851-6
id28229025
pubmed_id36042261
bacterial resistance :
Ampicillin
cloning :
backbonepDR111
backbone_mutationAddition of insert and fusion components to NheI site. Phyper-spank replaced by D-mannose promoter and lacI replaced by mannose regulator
backbone_origin
backbone_size8858
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
Expresses mouse transcription factors Stat-1 and Klf6 in an operon from D-mannose promoter for Bacillus subtilis. The secretion peptide PhoD is fused to both factors.
origin :
30
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3EagI
cloning_site_5SalI
promoterD-mannose
sequencing_primer_3TACCTCCTTgtcgactAAGCTT
sequencing_primer_5cggctagcTTACAGATGCCTT
site_3_destroyed
site_5_destroyed
entrez_gene
aliases2010005J02Rik
geneStat1
id20846
genbank_ids
mutation
nameStat-1
shRNA_sequence
size2250
species
10090
Mus musculus
32644
Other
Escherichia coli
tags
locationN terminal on insert
tagPhoD secretion peptide
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3NheI
cloning_site_5EagI
promoterD-mannose
sequencing_primer_3TACCTCCTTgtcgactAAGCTT
sequencing_primer_5cggctagcTTACAGATGCCTT
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAI448727, BCD1, C86813, CPBP, Copeb, FM2, FM6, Ierepo1, Ierepo3, R75280, Zf9
geneKlf6
id23849
genbank_ids
mutation
nameKlf6
shRNA_sequence
size850
species
10090
Mus musculus
tags
location
tagPhoD secretion peptide
resistance markers :
1092
tags :
High Copy
terms :
Other
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA