This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3-AKT-PH-GFP
catalog :
18836
citations: 21
Reference
Domma A, Henderson L, Goodrum F, Moorman N, Kamil J. Human cytomegalovirus attenuates AKT activity by destabilizing insulin receptor substrate proteins. J Virol. 2023;97:e0056323 pubmed publisher
Domma A, Goodrum F, Moorman N, Kamil J. Human cytomegalovirus attenuates AKT activity by destabilizing insulin receptor substrate proteins. bioRxiv. 2023;: pubmed publisher
Hu S, He X, Nie J, Hou J, Wu J, Liu X, et al. Methylene-bridge tryptophan fatty acylation regulates PI3K-AKT signaling and glucose uptake. Cell Rep. 2022;38:110509 pubmed publisher
Brandel A, Aigal S, Lagies S, Schlimpert M, Meléndez A, Xu M, et al. The Gb3-enriched CD59/flotillin plasma membrane domain regulates host cell invasion by Pseudomonas aeruginosa. Cell Mol Life Sci. 2021;78:3637-3656 pubmed publisher
Voss O, Gillespie J, Lehman S, Rennoll S, Beier Sexton M, Rahman M, et al. Risk1, a Phosphatidylinositol 3-Kinase Effector, Promotes Rickettsia typhi Intracellular Survival. MBio. 2020;11: pubmed publisher
Chen G, Park D, Magis A, Behera M, Ramalingam S, Owonikoko T, et al. Mcl-1 Interacts with Akt to Promote Lung Cancer Progression. Cancer Res. 2019;79:6126-6138 pubmed publisher
Ren C, Yuan Q, Braun M, Zhang X, Petri B, Zhang J, et al. Leukocyte Cytoskeleton Polarization Is Initiated by Plasma Membrane Curvature from Cell Attachment. Dev Cell. 2019;49:206-219.e7 pubmed publisher
Yang S, Zhang Y, Shen F, Ma X, Zhang M, Hou Y, et al. The flavonoid baicalin improves glucose metabolism by targeting the PH domain of AKT and activating AKT/GSK3β phosphorylation. FEBS Lett. 2018;: pubmed publisher
Iuliano O, Yoshimura A, Prospéri M, Martin R, Knölker H, Coudrier E. Myosin 1b promotes axon formation by regulating actin wave propagation and growth cone dynamics. J Cell Biol. 2018;217:2033-2046 pubmed publisher
Burda P, Caldelari R, Heussler V. Manipulation of the Host Cell Membrane during Plasmodium Liver Stage Egress. MBio. 2017;8: pubmed publisher
Hao F, Xu Q, Zhao Y, Stevens J, Young S, Sinnett Smith J, et al. Insulin Receptor and GPCR Crosstalk Stimulates YAP via PI3K and PKD in Pancreatic Cancer Cells. Mol Cancer Res. 2017;15:929-941 pubmed publisher
Rennoll Bankert K, Rahman M, Guillotte M, Lehman S, Beier Sexton M, Gillespie J, et al. RalF-Mediated Activation of Arf6 Controls Rickettsia typhi Invasion by Co-Opting Phosphoinositol Metabolism. Infect Immun. 2016;84:3496-3506 pubmed
Kimura S, Yamashita M, Yamakami Kimura M, Sato Y, Yamagata A, Kobashigawa Y, et al. Distinct Roles for the N- and C-terminal Regions of M-Sec in Plasma Membrane Deformation during Tunneling Nanotube Formation. Sci Rep. 2016;6:33548 pubmed publisher
Panda P, Behera B, Meher B, Das D, Mukhopadhyay S, Sinha N, et al. Abrus Agglutinin, a type II ribosome inactivating protein inhibits Akt/PH domain to induce endoplasmic reticulum stress mediated autophagy-dependent cell death. Mol Carcinog. 2017;56:389-401 pubmed publisher
Nakajima K, Zhu K, Sun Y, Hegyi B, Zeng Q, Murphy C, et al. KCNJ15/Kir4.2 couples with polyamines to sense weak extracellular electric fields in galvanotaxis. Nat Commun. 2015;6:8532 pubmed publisher
Piper M, Lee A, van Horck F, McNeilly H, Lu T, Harris W, et al. Differential requirement of F-actin and microtubule cytoskeleton in cue-induced local protein synthesis in axonal growth cones. Neural Dev. 2015;10:3 pubmed publisher
Gault W, Enyedi B, Niethammer P. Osmotic surveillance mediates rapid wound closure through nucleotide release. J Cell Biol. 2014;207:767-82 pubmed publisher
O Neill P, Gautam N. Subcellular optogenetic inhibition of G proteins generates signaling gradients and cell migration. Mol Biol Cell. 2014;25:2305-14 pubmed publisher
Ni Y, Sinnett Smith J, Young S, Rozengurt E. PKD1 mediates negative feedback of PI3K/Akt activation in response to G protein-coupled receptors. PLoS ONE. 2013;8:e73149 pubmed publisher
Yi K, Axtmayer J, Gustin J, Rajpurohit A, Lauring J. Functional analysis of non-hotspot AKT1 mutants found in human breast cancers identifies novel driver mutations: implications for personalized medicine. Oncotarget. 2013;4:29-34 pubmed
Kwon Y, Hofmann T, Montell C. Integration of phosphoinositide- and calmodulin-mediated regulation of TRPC6. Mol Cell. 2007;25:491-503 pubmed
product information
Catalog Number :
18836
Product Name :
pcDNA3-AKT-PH-GFP
article :
doi10.1016/j.molcel.2007.01.021
id2182
pubmed_id17317623
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3
backbone_mutation
backbone_originInvitrogen
backbone_size5000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5EcoRI
promoterCMV
sequencing_primer_3BGH-rev
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA
geneAKT1
id207
genbank_ids
mutationcontains amino acids 1-148 (compared to GenBank reference NP_005154.2)
nameAKT-PH domain
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagEGFP
resistance markers :
346
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA