dCas9-NLS-gs10-cLYN268 | Addgene 188259 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

dCas9-NLS-gs10-cLYN268

catalog :

188259

citations: 1

Reference
Rihtar E, Lebar T, Lain x161 x10d ek D, Kores K, Le x161 nik S, Bren U, et al. Chemically inducible split protein regulators for mammalian cells. Nat Chem Biol. 2023;19:64-71 pubmed publisher

product information

Catalog Number :

188259

Product Name :

dCas9-NLS-gs10-cLYN268

article :

doi	10.1038/s41589-022-01136-x
id	28229005
pubmed_id	36163385

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA3

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

CRISPR

growth strain :

Plasmid ensures constitutive expression of the C-terminal fragment of split LYN kinase domain (aa 239-267), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminus

origin :

pi :

alt_names

cloning

clone_method	Gibson Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	JTK8, SAIDV, p53Lyn, p56Lyn
gene	LYN
id	4067

genbank_ids

mutation

name

cLYN268

shRNA_sequence

size

species

9606

Homo sapiens