This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pN1-RnKIF5C(1-560, 6)-Halo-Flag
catalog :
188072
citations: 1
Reference
Budaitis B, Badieyan S, Yue Y, Blasius T, Reinemann D, Lang M, et al. A kinesin-1 variant reveals motor-induced microtubule damage in cells. Curr Biol. 2022;32:2416-2429.e6 pubmed publisher
product information
Catalog Number :
188072
Product Name :
pN1-RnKIF5C(1-560, 6)-Halo-Flag
article :
doi10.1016/j.cub.2022.04.020
id28228981
pubmed_id35504282
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-N1
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Mammalian cell expression of truncated version (aa 1-560) of rat kinesin-1 KIF5C tagged with Halo and Flag tags. The delta6 mutation removes the first 6 amino acids.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3AgeI
cloning_site_5HindIII
promoterCMV
sequencing_primer_3
sequencing_primer_5EGFPN1seqfor
site_3_destroyed
site_5_destroyed
entrez_gene
aliases
geneKif5c
id311024
genbank_ids
mutationaa1-560, deletion of aa2-6
namerat KIF5C
shRNA_sequence
size
species
10116
Rattus norvegicus
tags
locationC terminal on insert
tagHalo
locationC terminal on insert
tagFlag
resistance markers :
2210
tags :
Unknown
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA