This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLKO.1 puro shRNA beta-catenin
catalog :
18803
citations: 21
Reference
Wan C, Mahara S, Sun C, Doan A, Chua H, Xu D, et al. Genome-scale CRISPR-Cas9 screen of Wnt/β-catenin signaling identifies therapeutic targets for colorectal cancer. Sci Adv. 2021;7: pubmed publisher
Wang W, Zhao Z, Han S, Wu D. miR-637 Prevents Glioblastoma Progression by Interrupting ZEB2/WNT/β-catenin Cascades. Cell Mol Neurobiol. 2021;: pubmed publisher
Liu J, Zeng S, Wang Y, Yu J, Ouyang Q, Hu L, et al. Essentiality of CTNNB1 in Malignant Transformation of Human Embryonic Stem Cells under Long-Term Suboptimal Conditions. Stem Cells Int. 2020;2020:5823676 pubmed publisher
Khare V, Tabassum S, Chatterjee U, Chatterjee S, Ghosh M. RNA helicase p68 deploys β-catenin in regulating RelA/p65 gene expression: implications in colon cancer. J Exp Clin Cancer Res. 2019;38:330 pubmed publisher
Hou X, Khan M, Turmaine M, Thrasivoulou C, Becker D, Ahmed A. Wnt signaling regulates cytosolic translocation of Connexin 43. Am J Physiol Regul Integr Comp Physiol. 2019;: pubmed publisher
Qiao Y, Wang J, Karagoz E, Liang B, Song X, Shang R, et al. Axis inhibition protein 1 (Axin1) Deletion-Induced Hepatocarcinogenesis Requires Intact β-Catenin but Not Notch Cascade in Mice. Hepatology. 2019;: pubmed publisher
You C, Zhang S, Sun Y, Zhang S, Tang G, Tang F, et al. β-catenin decreases acquired TRAIL resistance in non-small-cell lung cancer cells by regulating the redistribution of death receptors. Int J Oncol. 2018;53:2258-2268 pubmed publisher
Kim J, Lee J, Hwang H, Lee D, Park C, Jung S, et al. Wnt signal activation induces midbrain specification through direct binding of the beta-catenin/TCF4 complex to the EN1 promoter in human pluripotent stem cells. Exp Mol Med. 2018;50:24 pubmed publisher
Bagati A, Bianchi Smiraglia A, Moparthy S, Kolesnikova K, Fink E, Kolesnikova M, et al. FOXQ1 controls the induced differentiation of melanocytic cells. Cell Death Differ. 2018;25:1040-1049 pubmed publisher
Sulaiman A, McGarry S, Li L, Jia D, Ooi S, Addison C, et al. Dual inhibition of Wnt and Yes-associated protein signaling retards the growth of triple-negative breast cancer in both mesenchymal and epithelial states. Mol Oncol. 2018;12:423-440 pubmed publisher
Kidan N, Khamaisie H, Ruimi N, Roitman S, Eshel E, Dally N, et al. Ectopic Expression of Snail and Twist in Ph+ Leukemia Cells Upregulates CD44 Expression and Alters Their Differentiation Potential. J Cancer. 2017;8:3952-3968 pubmed publisher
Cho S. APC downregulated 1 inhibits breast cancer cell invasion by inhibiting the canonical WNT signaling pathway. Oncol Lett. 2017;14:4845-4852 pubmed publisher
Nagaraj A, Wang Q, Joseph P, Zheng C, Chen Y, Kovalenko O, et al. Using a novel computational drug-repositioning approach (DrugPredict) to rapidly identify potent drug candidates for cancer treatment. Oncogene. 2018;37:403-414 pubmed publisher
Bagati A, Bianchi Smiraglia A, Moparthy S, Kolesnikova K, Fink E, Lipchick B, et al. Melanoma Suppressor Functions of the Carcinoma Oncogene FOXQ1. Cell Rep. 2017;20:2820-2832 pubmed publisher
Vergara D, Stanca E, Guerra F, Priore P, Gaballo A, Franck J, et al. ?-Catenin Knockdown Affects Mitochondrial Biogenesis and Lipid Metabolism in Breast Cancer Cells. Front Physiol. 2017;8:544 pubmed publisher
Sulaiman A, Sulaiman B, Khouri L, McGarry S, Nessim C, Arnaout A, et al. Both bulk and cancer stem cell subpopulations in triple-negative breast cancer are susceptible to Wnt, HDAC, and ER? coinhibition. FEBS Lett. 2016;590:4606-4616 pubmed publisher
Santos J, Carrasco Garcia E, Garcia Puga M, Aldaz P, Montes M, Fernández Reyes M, et al. SOX9 Elevation Acts with Canonical WNT Signaling to Drive Gastric Cancer Progression. Cancer Res. 2016;76:6735-6746 pubmed publisher
Nagaraj A, Joseph P, Kovalenko O, Singh S, Armstrong A, Redline R, et al. Critical role of Wnt/β-catenin signaling in driving epithelial ovarian cancer platinum resistance. Oncotarget. 2015;6:23720-34 pubmed
Morgan R, Pearn L, Liddiard K, Pumford S, Burnett A, Tonks A, et al. ?-Catenin is overexpressed in acute myeloid leukemia and promotes the stabilization and nuclear localization of ?-catenin. Leukemia. 2013;27:336-43 pubmed publisher
Ma B, van Blitterswijk C, Karperien M. A Wnt/?-catenin negative feedback loop inhibits interleukin-1-induced matrix metalloproteinase expression in human articular chondrocytes. Arthritis Rheum. 2012;64:2589-600 pubmed publisher
Onder T, Gupta P, Mani S, Yang J, Lander E, Weinberg R. Loss of E-cadherin promotes metastasis via multiple downstream transcriptional pathways. Cancer Res. 2008;68:3645-54 pubmed publisher
product information
Catalog Number :
18803
Product Name :
pLKO.1 puro shRNA beta-catenin
article :
doi10.1158/0008-5472.CAN-07-2938
id2176
pubmed_id18483246
bacterial resistance :
Ampicillin
cloning :
backbonepLKO.1 puro
backbone_mutation
backbone_originAvailable at Addgene (#8453)
backbone_size7000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
RNAi
growth notes :
siRNA directed against beta-catenin: 5'-GCTTGGAATGAGACTGCTGAT-3'. Sequence from the website of the RNAi consortium at the Broad institute.
growth strain :
3rd gen lentiviral vector for knocking down beta-catenin gene expression
origin :
37
pi :
alt_names
beta-catenin
CTNNB1
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5AgeI
promoterU6
sequencing_primer_3
sequencing_primer_5LKO.1 5'
site_3_destroyed
site_5_destroyed1
entrez_gene
aliasesCTNNB, EVR7, MRD19, NEDSDV, armadillo
geneCTNNB1
id1499
genbank_ids
mutation
nameb-catenin shRNA
shRNA_sequenceGCTTGGAATGAGACTGCTGAT
size
species
9606
Homo sapiens
tags
resistance markers :
568
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA