pLKO.1 hygro shRNA E-cadherin | Addgene 18802 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pLKO.1 hygro shRNA E-cadherin

catalog :

18802

citations: 2

Reference
Tanaka S, Aoyama T, Ogawa M, Takasawa A, Murata M, Osanai M, et al. Cytotoxicity of Clostridium perfringens enterotoxin depends on the conditions of claudin-4 in ovarian carcinoma cells. Exp Cell Res. 2018;: pubmed publisher
Onder T, Gupta P, Mani S, Yang J, Lander E, Weinberg R. Loss of E-cadherin promotes metastasis via multiple downstream transcriptional pathways. Cancer Res. 2008;68:3645-54 pubmed publisher

product information

Catalog Number :

18802

Product Name :

pLKO.1 hygro shRNA E-cadherin

article :

doi	10.1158/0008-5472.CAN-07-2938
id	2176
pubmed_id	18483246

bacterial resistance :

Ampicillin

cloning :

backbone

pLKO.1 hygro

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

RNAi

growth notes :

siRNA directed against E-cadherin: 5'-AAGATAGGAGTTCTCTGATGC-3'. Sequence from the website of the RNAi consortium at the Broad institute. Please note that the sequence originally published in the paper was incorrect and the sequence above is the actual shRNA used.

origin :

pi :

alt_names

E-Cadherin

cloning

clone_method	Restriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5	LKO.1 5'
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	Arc-1, BCDS1, CD324, CDHE, ECAD, LCAM, UVO
gene	CDH1
id	999

genbank_ids

mutation

name

E-cadherin shRNA

shRNA_sequence

size

species

9606

Homo sapiens