pLKO.1 puro shRNA E-cadherin | Addgene 18801 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pLKO.1 puro shRNA E-cadherin

catalog :

18801

citations: 8

Reference
Aouad P, Zhang Y, De Martino F, Stibolt C, Ali S, Ambrosini G, et al. Epithelial-mesenchymal plasticity determines estrogen receptor positive breast cancer dormancy and epithelial reconversion drives recurrence. Nat Commun. 2022;13:4975 pubmed publisher
Ouyang H, Luong P, Frodin M, Hansen S. p190A RhoGAP induces CDH1 expression and cooperates with E-cadherin to activate LATS kinases and suppress tumor cell growth. Oncogene. 2020;: pubmed publisher
Scott A, Walker S, Krank J, Wilkinson A, Johnson K, Lewis E, et al. AIF promotes a JNK1-mediated cadherin switch independently of respiratory chain stabilization. J Biol Chem. 2018;293:14707-14722 pubmed publisher
Li X, Zhao Z, Zhang X, Yang S, Lin X, Yang X, et al. Klf4 reduces stemness phenotype, triggers mesenchymal-epithelial transition (MET)-like molecular changes, and prevents tumor progression in nasopharygeal carcinoma. Oncotarget. 2017;8:93924-93941 pubmed publisher
Liu X, Zhou L, Hu J, Liu L, Wan H, Zhang X. UNC0638, a G9a inhibitor, suppresses epithelial?mesenchymal transition?mediated cellular migration and invasion in triple negative breast cancer. Mol Med Rep. 2018;17:2239-2244 pubmed publisher
Petrova Y, Schecterson L, Gumbiner B. Roles for E-cadherin cell surface regulation in cancer. Mol Biol Cell. 2016;27:3233-3244 pubmed
Maiden S, Petrova Y, Gumbiner B. Microtubules Inhibit E-Cadherin Adhesive Activity by Maintaining Phosphorylated p120-Catenin in a Colon Carcinoma Cell Model. PLoS ONE. 2016;11:e0148574 pubmed publisher
Onder T, Gupta P, Mani S, Yang J, Lander E, Weinberg R. Loss of E-cadherin promotes metastasis via multiple downstream transcriptional pathways. Cancer Res. 2008;68:3645-54 pubmed publisher

product information

Catalog Number :

18801

Product Name :

pLKO.1 puro shRNA E-cadherin

article :

doi	10.1158/0008-5472.CAN-07-2938
id	2176
pubmed_id	18483246

bacterial resistance :

Ampicillin

cloning :

backbone

pLKO.1 puro

backbone_mutation

backbone_origin

Available at Addgene (#8453)

backbone_size

7000

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

RNAi

growth notes :

siRNA directed against E-cadherin: 5'-AAGATAGGAGTTCTCTGATGC-3'. Sequence from the website of the RNAi consortium at the Broad institute. Please note that the sequence originally published in the paper was incorrect and the sequence above is the actual shRNA used.

origin :

pi :

alt_names

E-Cadherin

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRI
cloning_site_5	AgeI
promoter	U6
sequencing_primer_3
sequencing_primer_5	LKO.1 5'
site_3_destroyed
site_5_destroyed	1

entrez_gene

aliases	Arc-1, BCDS1, CD324, CDHE, ECAD, LCAM, UVO
gene	CDH1
id	999

genbank_ids

mutation

name

E-cadherin shRNA

shRNA_sequence

AAGATAGGAGTTCTCTGATGC

size

species

9606

Homo sapiens