pWZL Blast Snail ER | Addgene 18798 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pWZL Blast Snail ER

catalog :

18798

citations: 10

Reference
Ware K, Thomas B, Olawuni P, Sheth M, Hawkey N, Yeshwanth M, et al. A synthetic lethal screen for Snail-induced enzalutamide resistance identifies JAK/STAT signaling as a therapeutic vulnerability in prostate cancer. Front Mol Biosci. 2023;10:1104505 pubmed publisher
Lin C, Yang W, Lin Y, Tang X, Chen P, Ding C, et al. DDR2 upregulation confers ferroptosis susceptibility of recurrent breast tumors through the Hippo pathway. Oncogene. 2021;40:2018-2034 pubmed publisher
Adachi Y, Ito K, Hayashi Y, Kimura R, Tan T, Yamaguchi R, et al. Epithelial-to-Mesenchymal Transition is a Cause of Both Intrinsic and Acquired Resistance to KRAS G12C Inhibitor in KRAS G12C-Mutant Non-Small Cell Lung Cancer. Clin Cancer Res. 2020;26:5962-5973 pubmed publisher
Frey P, Devisme A, Schrempp M, Andrieux G, Boerries M, Hecht A. Canonical BMP Signaling Executes Epithelial-Mesenchymal Transition Downstream of SNAIL1. Cancers (Basel). 2020;12: pubmed publisher
Grisard E, Coan M, Cesaratto L, Rigo I, Zandonà L, Paulitti A, et al. Sleeping beauty genetic screen identifies miR-23b::BTBD7 gene interaction as crucial for colorectal cancer metastasis. EBioMedicine. 2019;46:79-93 pubmed publisher
Xu J, Zhang W, Tang L, Chen W, Guan X. Epithelial-mesenchymal transition induced PAI-1 is associated with prognosis of triple-negative breast cancer patients. Gene. 2018;670:7-14 pubmed publisher
Kidan N, Khamaisie H, Ruimi N, Roitman S, Eshel E, Dally N, et al. Ectopic Expression of Snail and Twist in Ph+ Leukemia Cells Upregulates CD44 Expression and Alters Their Differentiation Potential. J Cancer. 2017;8:3952-3968 pubmed publisher
Ware K, Somarelli J, Schaeffer D, Li J, Zhang T, Park S, et al. Snail promotes resistance to enzalutamide through regulation of androgen receptor activity in prostate cancer. Oncotarget. 2016;7:50507-50521 pubmed publisher
Salt M, Bandyopadhyay S, McCormick F. Epithelial-to-mesenchymal transition rewires the molecular path to PI3K-dependent proliferation. Cancer Discov. 2014;4:186-99 pubmed publisher
Mani S, Guo W, Liao M, Eaton E, Ayyanan A, Zhou A, et al. The epithelial-mesenchymal transition generates cells with properties of stem cells. Cell. 2008;133:704-15 pubmed publisher

product information

Catalog Number :

18798

Product Name :

pWZL Blast Snail ER

article :

doi	10.1016/j.cell.2008.03.027
id	2175
pubmed_id	18485877

bacterial resistance :

Ampicillin

cloning :

backbone

pWZL-Blast

backbone_mutation

backbone_origin

backbone_size

5100

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Retroviral

growth notes :

pWZL-Blast-Snail-ER was generated by replacing the MYC cDNA of pWZL-Blast-DN-MycER (Littlewood et al., 1995; Watnick et al., 2003) with the hSnail cDNA PCR amplified from pBp-hSnail.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	BamHI
cloning_site_5	BamHI
promoter
sequencing_primer_3	IRES-R (5'-GCATTCCTTTGGCGAGAG-3')
sequencing_primer_5	pBABE 5'
site_3_destroyed	1
site_5_destroyed

entrez_gene

aliases	SLUGH2, SNA, SNAH, SNAIL, SNAIL1, dJ710H13.1
gene	SNAI1
id	6615

genbank_ids

mutation

G525R mutation in the ER. The presence of the G525R point mutation in the cloned ER sequence abrogates its binding to 17 -estradiol while keeping its full sensitivity to the synthetic ligand 4'hydroxytamoxifen (4OHT).

name

snail

shRNA_sequence

size

species

9606

Homo sapiens