pFastBac_Dual_GST-TEV-EGFP-TBK1 | Addgene 187830 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pFastBac_Dual_GST-TEV-EGFP-TBK1

catalog :

187830

citations: 1

Reference
Nguyen T, Sawa Makarska J, Khuu G, Lam W, Adriaenssens E, Fracchiolla D, et al. Unconventional initiation of PINK1/Parkin mitophagy by Optineurin. Mol Cell. 2023;83:1693-1709.e9 pubmed publisher

product information

Catalog Number :

187830

Product Name :

pFastBac_Dual_GST-TEV-EGFP-TBK1

article :

doi	10.1016/j.molcel.2023.04.021
id	28228930
pubmed_id	37207627

bacterial resistance :

Ampicillin

cloning :

backbone

pFastBac_Dual

backbone_mutation

backbone_origin

Thermofisher Scientific

backbone_size

5238

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

Insect Expression

growth notes :

Codon Optimized for Sf9 Expression

growth strain :

Plasmid for the expression and purification of GST-TEV-EGFP-TBK1 in Spodoptera frugiperda cells (Sf9). Internal reference: SMC1631

origin :

pi :

IIAE8, NAK, T2KgeneTBK1id29110genbank_ids

29110

NM_013254

mutationnameTBK1shRNA_sequencesize3625species

9606

Homo sapiens

tags

location	N terminal on backbone
tag	GST

location	N terminal on backbone
tag	TEV-mEGFP-GG Linker

Cloningcloning_site_3
cloning_site
_5promotertd>sequencing_pri
mer_3CCTCTAGTACTTCTCGACAAGCTTT
TACAGACAGTCCACGTTTCTCAGT<
td>sequencing_primer_5GTGGAGGC
ATGCAATCCACATCCAACCACC>site_3_destroyed>site_5_destroyed>entrez_gene<
/td>

a
liases

FTDALS4,

alt_names

NAK

cloning

clone_method

Gibson

resistance markers :

5424

tags :

Unknown