MSCV IRES Luciferase | Addgene 18760 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

MSCV IRES Luciferase

catalog :

18760

citations: 3

Reference
Takeda M, Sakamoto H, Shibasaki N, Fukui T, Magaribuchi T, Sumiyoshi T, et al. Extracellular vesicles secreted from bone metastatic renal cell carcinoma promote angiogenesis and endothelial gap formation in bone marrow in a time-dependent manner in a preclinical mouse model. Front Oncol. 2023;13:1139049 pubmed publisher
Rappazzo C, Tse L, Kaku C, Wrapp D, Sakharkar M, Huang D, et al. An Engineered Antibody with Broad Protective Efficacy in Murine Models of SARS and COVID-19. bioRxiv. 2020;: pubmed publisher
Bechill J, Zhong R, Zhang C, Solomaha E, Spiotto M. A High-Throughput Cell-Based Screen Identified a 2-[(E)-2-Phenylvinyl]-8-Quinolinol Core Structure That Activates p53. PLoS ONE. 2016;11:e0154125 pubmed publisher

product information

Catalog Number :

18760

Product Name :

MSCV IRES Luciferase

article :

doi
id	2161
pubmed_id

bacterial resistance :

Ampicillin

cloning :

backbone

MSCV IRES Luciferase

backbone_mutation

backbone_origin

backbone_size

6300

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Retroviral

Luciferase

growth notes :

To create MSCV/IRES/LUC, the MSCV/IRES/GFP plasmid was amplified up and digested with NcoI and SalI to remove the GFP. Next, the pGL3-Promoter vector from Promega was amplified up and digested with NcoI and SalI to remove luciferase and the Poly(A) signal. Once completed, the 1.7kb luciferase was ligated into MSCV/IRES to give the MSCV/IRES/LUC plasmid.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5	MSCV
site_3_destroyed
site_5_destroyed

entrez_gene

genbank_ids

mutation

name

shRNA_sequence

size

species